CU-Boulder PSYC 2841 - Unit 4 Course Packet Key (9 pages)

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Unit 4 Course Packet Key



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Unit 4 Course Packet Key

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9
School:
University of Colorado at Boulder
Course:
Psyc 2841 - Independent Study (Lower Division)
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MCDB 2150 Principles of Genetics Course Packet Fall 2017 Unit 4 Applications of Genetics Class 31 Gene Interactions Complementation and Epistasis 1 Three true breeding mutants with the same disease phenotype were isolated When crossed mutants 1 and 2 showed complementation and mutations 1 and 3 showed non complementation A Fill in the pedigrees below to reflect this information B Using capital and small letters to represent different alleles write the genotype of each individual in the blank beside the symbol You can choose which letter s to use C If the female and male from generation 2 of the two pedigrees above were to have offspring what percent of their offspring would show the mutant phenotype AaBb X aaBB 50 2 You are studying the genetics of eye formation in fruit fly 5 different mutant strains of fly have been isolated A E and each shows a recessive eye absent trait i e very small or no eye Crosses were performed as follows to test complementation and produced progeny with wild type eyes or eye absent A B C D E A B C D E A Based on the information in the table at least how many genes are needed to make an eye in fly 2 B Which flies have a mutation in the same gene are in the same complementation group ABE DC MCDB 2150 Principles of Genetics Course Packet Fall 2017 3 Autosomal recessive mutations in two different genes cause worms to produce defective eggs One mutant ee lays mis shapen eggs The other mutant qq lays dead eggs You cross together a mutant worm that lays mis shapen eggs with a mutant that lays dead eggs The offspring of this cross all can lay normal eggs You cross the F1 progeny with each other and find 27 worms that lay normal eggs 8 that lay mis shapen eggs and 15 that lay dead eggs A What is the ratio of phenotypes What inheritance pattern does this ratio suggest What can you conclude about the interaction between the two gene products 9 normal eggs 4 dead eggs 3 mis shapen eggs recessive epistasis Q is epistatic to E B What are the genotypes of the F2 progeny 27 normal eggs E Q 8 mis shapen eggs eeQ 15 dead eggs E qq and eeqq Class 32 Developmental Genetics I Maternal and Cytoplasmic Inheritance 1 Fill in generations 2 and 3 on two pedigrees below so that they are consistent with their modes of inheritance Use a if you are unsure of the phenotype The female in the third generation would not be colored in because her mom has to be Aa The son in generation 3 is because we do not know his mother s genotype MCDB 2150 Principles of Genetics Course Packet Fall 2017 2 In worms protein A activates Protein B Protein B when active inhibits apoptosis Protein A is inactivated by phosphorylation A Draw the pathway for apoptosis involving proteins A and B based on the description above B A mutation in the promoter region of the gene for protein B prevents the gene from being transcribed What is the phenotype of this mutation apoptosis is not inhibited more cell death B is not made so apoptosis is not inhibited Is this mutation a gain of function or loss of function mutation Explain LOF because the protein does not function at all it is not present C A mutation in the gene that codes for protein A does not allow protein A to be phosphorylated What is the phenotype of this mutation A is never inhibited B is always active apoptosis is extra inhibited less cell death Is this mutation a gain of function or loss of function mutation Explain GOF because the protein gained the new function of always being active Class 33 Developmental Genetics II Genes and Effects of Mutations 1 List the main function for each class of early development genes in drosophila from the functions listed below Then from the choices listed below A D select what the correct phenotype for a mutation in each class of genes functions define anterior and posterior of embryo define anterior and posterior of a segment define segment boarders A Improper orientation of body and wing hairs B Head and thorax are missing segments 7 and 8 are fused C embryo lacks posterior structures MCDB 2150 Principles of Genetics Course Packet Fall 2017 D deletion of a region of every other segment Class 34 Epigenetics I Methylation and Imprinting 1 You are studying the epigenetic patterns at the gene YYX in rats This gene is responsible for synapse formation during early brain development You take newborn genetically identical rats and place them under the care of distinctly different mother rats The A mother is very nurturing and constantly licks her surrogate brood in contrast to mother B who largely ignores the infants After several days you isolate brain tissue from the rats being raised by each mother First you isolate DNA from the promoter region of the gene of interest and treat half of each sample with sodium bisulfite A Bisulfite converts unmethylated C to U T You obtain the following results file from the sequencing facility Rat Mother Treated A CGTATGTTATGA B CGTACGTTACGA Untreated CGTACGTTACGA CGTACGTTACGA B How many cytosine bases are methylated in the B mouse 3 Circle the methylated bases in the sequence from mouse B below CGTACGTTACGA C How many cytosine bases are methylated in the A mouse 1 Circle the methylated bases in the sequence from mouse B below CGTACGTTACGA C In the rat from which parent would you expect to see greater transcription of the YYX gene Rat A Explain your answer Rat A has less methylation in the promoter region of the gene so it is likely transcribed more leading to increased synapse formation MCDB 2150 Principles of Genetics Course Packet Fall 2017 2 The gene H gene is involved in brain development When absent individuals have developmental defects H is differentially methylated in the male germline H is methylated while in the female germline H is unmethylated The pedigree shows a family carrying a deletion of the H gene Unaffected carriers grey symbols have one deleted copy of the gene and one normal copy of the gene They also have an unmethylated H gene Affected individuals black symbols have a copy of the deletion and also have a methylated H gene Unaffected non carriers unfilled symbols do not have the H deletion Using the following notation H represents a chromosome with an unmethylated H gene H represents a chromosome with a methylated H gene represents a chromosome with a deletion of the H gene A What are the genotypes of the following individuals 1 H 2 HH 3 H B Individual 4 has the disorder even though his father is wild type at the H locus Why does he have the disorder He got a


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