Wright BIO 2110 - BIO2110_f16_exam1 (14 pages)

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BIO2110_f16_exam1



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BIO2110_f16_exam1

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Pages:
14
School:
Wright State University
Course:
Bio 2110 - Principles of Molecular and Classical Genetics
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Name BIO 2110 Genetis Exam 1 30 September 2016 see last page for potentially useless information Name Name 1 In eukaryotic cells what happens when the leading strand from one origin of replication meets the lagging strand from an adjacent origin of replication Name 2 Below is a diagram of a eukaryotic protein coding gene Numbered boxes represent exons Jagged lines represent introns Start ATG and stop TGA codons of the openreading frame are indicated Indicate on this diagram as precisely as possible the positions of i the promoter ii the polyA addition signal iii the 5 UTR and iv the 3 UTR v What impact s would a mutation that deleted the polyA addition signal have on the transcription AND OR co transcriptional processing of RNA from this gene Explain the reasoning behind your answer s ATG TGA 1 2 3 Name 3 Chromatin structure can impact gene expression see below In closed chromatin genes can not be transcribed In open chromatin genes can be transcribed Describe a transcriptional regulatory mechanism that can convert closed chromatin to open chromatin This mechanism includes three steps Include all of these steps in your answer Name 4 Below is a diagram of a eukaryotic protein coding gene Exons numbered 1 4 are shown as thick horizontal lines Flanking DNA is shown as thin horizontal lines The position of the start codon in exon 1 is indicated by an arrowhead Below this diagram is a partial sequence of the coding strand of this gene that includes the start codon and that spans intron 1 Exonic sequences are indicated with upper case letters Intronic sequences are indicated with lower case letters 1 2 3 4 AGTGAATGGTAAAGguaagtttcttctgatcattagatggactaccgatcagTTTACATTT i What are the first five amino acids that are coded for in this gene Use the genetic code chart included on the last page of this exam ii Which of the following mutations most likely would have a deleterious impact on the function of this gene Explain your answer an A T substitution at position 3 in codon two deletion of all three nucleotides of codon two deletion the second nucleotide of codon two Name 5 What role does the 5 cap play in mRNA stability in eukaryotic mRNAs What roles does the 5 cap play in protein synthesis in eukaryotic cells Name 6 What is the branch point nucleotide of a eukaryotic intron Where is the branch point nucleotide located What role does the branch point nucleotide play in the splicing of introns Name 7 In the nematode C elegans the erztz gene is expressed in the excretory gland and the post anal gland see below This expression pattern also is observed in GFP reporter genes that contain 5 kb of 5 flanking DNA reporter gene A below Some deletions within this region of 5 flanking DNA dotted lines in C D alter this expression pattern i Based on these deletions circle the regions of 5 flanking DNA contain enhancers that regulate expression in the excretory excr and or post anal pag gland indicate which region regulates expression in which gland ii Would the enhancers in these regions of DNA be the same or different Explain your answer iii Based on these results how must the cells of the excretory gland differ from the cells of the anal gland in regard to regulation of erztz gene transcription excretory gland post anal gland excr erztz 5 5lanking DNA pag A GFP B GFP C GFP D GFP Name 8 Glutamine Gln is coded for by two codons CAA and CAG A single tRNA with an anticodon of 5 UUG 3 can read translate both of these codon If the tRNAgln anticodon was mutated from 5 UUG 3 to 5 GUG 3 i Would the mutant tRNA be charged with Gln or with some other amino acid If different which other amino acid Explain your answer in the context of substrate specificity of aminoacyl tRNA synthetases ii Would the mutant tRNA still be able to read CAA and CAG codons If not which if any codon s would the mutant tRNA be able to read Name 9 Transcriptional activators have two distinct functional domains What are the functions of these two domains Name 10 In eukaryotic protein coding genes the stop codon always is in the last exon Provide a mechanistic reason to explain why this essential Name Name


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