Name ________________________ BIO 2110 - Genetics Exam 1 26 September 2014 Ten Questions / Ten Points per QuestionName ________________________Name ________________________ 1. Shown below is a diagram of a nucleotide monophosphate. Is this diagram of a ribonucleotide or a deoxyribonucleotide? Explain your answer. Also, on the diagram, indicate the positions of the 5’ and 3’ carbons. --DNA deoxyribonucleotides versus RNA ribonucleotides. In ribonucleotides, the sugar component is ribose while in deoxyribonucleotides, the sugar component is deoxyribose. Instead of a hydroxyl group at the second carbon in the ribose ring, it is replaced by a hydrogen atom.1’2’3’4’5’Name ________________________ 2. Below are shown the sequences of two strands of DNA from a protein-coding gene. The translation start site is included in the sequences shown. Based on these sequences, what would be the first four amino acids coded for in this protein? 5’ A G G C G T A C C G C A G T G T C C T T A G C A T T A C G 3’ 3’ T C C G C A T G G C G T C A C A G G A A T C G T A A T G C 5’Name ________________________ 3. Describe the two chemical reactions that are involved in the removal of introns from a pol II transcript.Name ________________________ 4. How does glucose regulate expression of the lac operon of E. coli? How does lactose regulate expression of the lac operon of E. coli?Name ________________________ 5. Polyadenylation is triggered by the presence of an UUAUUU sequence on an RNA polymerase II transcript. What happens to the transcript when this sequence emerges from the polymerase? (be as complete as possible)Name ________________________ 6. Which amino acid residue in the C-terminal repeat of RNA polymerase II is phosphorylated while pol II is at the promoter? Which amino acid residue is phosphylated during transcription elongation? How does phosphylation of these amino acid residues facilitate co-transcription processing of pol II transcripts?Name ________________________ 7. What enzymes are involved in the processing of pre-miRNAs into miRNAs? Where in the cell do each of these enzymes act? Which of these enzymes are involved in the processing of exogenous dsRNA into siRNAs?Name ________________________ 8. Transcriptional activators such as GAL4 have two distinct domains. What are the functions of each of these domains? Describe two ways by which transcriptional activators can facilitate the formation of a transcription initiation complex.Name ________________________ 9. What structure does eIF4 bind to? How does the binding of eIF4 to this structure facilitate the initiation of protein synthesis?Name ________________________ 10. Below is shown a diagram of a eukaryotic gene. Exons 2A and 2B are alternatively spliced in different cell types. On or below this diagram: i. indicate the site(s) RNA polymerase II would bind to during the formation of a transcription initiation complex. ii. indicate the site(s) transcriptional activators would bind to iii. draw the structure of the mRNA(s) that could result from transcription of this gene. Include post-transcriptional modifications in your drawing(s). 2A 2B 31enhancerpromoterexonintronunspecified region of
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