Wright BIO 2110 - Rec9_sequence conservation (2 pages)

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Rec9_sequence conservation



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Rec9_sequence conservation

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2
School:
Wright State University
Course:
Bio 2110 - Principles of Molecular and Classical Genetics
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BIO 2110 Recitation 9 17 November 2017 Name Names of group members 1 Below is shown a partial sequence of a highly conserved protein coding gene Also shown are the amino acids that are coded for by this DNA sequence Mutations that change any any of the amino acids in this sequence result in complete loss of protein function and are recessive lethal mutations Of the three nucleotides indicated by arrowheads the G in the Ser codon the C in the Arg codon the A in the Leu codon i which would be most highly conserved least variation ii which would be least highly conserved most variation iii explain you answers for i ii ATG TGG AGC CGA CAG CTA Met Trp Ser Arg Gln Leu 2 In the diagram below the positions of ortholog pairs are plotted on the C elegans Yaxis and C briggsae X axis X chromosomes i knowing the sequences of both genomes how would you identifiy pairs of orthologs ii what assumption is made in sequence based identification of orthologs iii what would this plot look like if the X chromosomes of C elegans and C briggsae were identical iv Groups of orthologous genes tend to form diagonal lines on this plot These lines can have an upward slope e g line A or a downward slope e g line B How would an upward vs a downward slope be interpreted in regard to the evolution of the X chromosome in this genus


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