BIO$2110$–$Genetics$ $ $ $ $ $ Name$_________________________________$Recitation$2$–$Genetic$Code$ $ $ $ $$Group$Members$________________________$ ________________________$$ ________________________$$$$Below$is$a$diagram$of$a$eukaryotic$gene.$$Exons$(numbered$1$–$4)$are$shown$as$thick$horizontal$lines.$$Flanking$DNA$is$shown$as$thin$horizontal$lines.$$The$position$of$the$start$codon$in$exon$1$is$indicated$by$an$arrowhead.$$Below$this$diagram$is$a$partial$sequence$of$this$gene$that$includes$the$start$codon$and$that$spans$intron$1.$$Exonic$sequences$are$indicated$with$upper$case$letters.$$Intronic$sequences$are$indicated$with$lower$case$letters.$$$$AGTGAATGATAAAGguaagtttcttctgatcattagatggactaccgatcagCTAACATTT 1.$ What$are$the$first$five$amino$acids$that$are$coded$for$in$this$gene?$$[Use$the$genetic$code$chart$included$on$the$last$page$of$this$recitation.$$Make$sure$you$start$at$the$start$codon.]$$$$$$$How$would$the$following$mutations$impact$the$coding$potential$of$this$gene?$$$2.$ A$two$bp$insertion$before$the$start$codon?$$AGTCTGAATGATAAAGguaagtttcttctgatcattagatggactaccgatcagCTAACATTT $$$$$$$3.$ A$one$bp$insertion$in$codon$two?$$$AGTGAATGAATAAAGguaagtttcttctgatcattagatggactaccgatcagCTAACATTT $$$$$$$$1234$4.$ A$two$bp$insertion$in$codon$two?$$$AGTGAATGAAATAAAGguaagtttcttctgatcattagatggactaccgatcagCTAACATTT $$$$$$5.$ An$AàT$substitution$at$position$3$of$exon$2?$$AGTGAATGATTAAGguaagtttcttctgatcattagatggactaccgatcagCTAACATTT $$$$$$$6.$ An$AàT$substitution$at$position$1$of$exon$2?$$AGTGAATGTTAAAGguaagtttcttctgatcattagatggactaccgatcagCTAACATTT $$$$$$$7.$ An$11$bp$deletion$at$the$3’$end$of$intron$1?$$AGTGAATGATAAAGguaagtttcttctgatcattagatggaCTAACATTT $$$$$$$8.$ Which$of$these$mutations$are$most$likely$to$be$null$mutations?$$[i.e.$to$eliminate$gene$function.]$
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