BIO$2110$–$Genetics$ $ $ $ $ $ Name$_________________________________$Recitation$1$–$Regulation$of$even-skipped$gene$expression$ $ $ $ $$Group$Members$________________________$ ________________________$$ ________________________$$$$The$even-skipped$(eve)$gene$is$expressed$in$seven$distinct$stripes$in$D.,melanogaster$embryos.$$Regulation$of$this$expression$is$modular,$i.e.$regulatory$modules$for$each$stripe$are$located$at$different$positions$in$the$eve$gene$(see$below).$$$$$eve$expression$pattern$$Structure$of$the$eve$gene.$$Positions$of$regulatory$are$$indicated$by$stripe$number.$$1.$ Explain$why$these$different$regulatory$modules$would$cause$LacZ$to$be$expressed$in$different$regions$of$the$embryo.$$i.e.,$i.$$$ How$do$these$modules$differ$from$each$other?$and$ii.$ How$do$the$different$regions$of$the$embryo$in$which$stripes$appear$differ$from$each$other?$$$ $2.$ The$diagrams$below$show$LacZ$expression$patterns$obtained$from$two$different$eve$reporter$genes.$$Draw$the$structures$of$the$ rep orter$genes$that$would$produce$these$patterns$of$LacZ$expression.$$Clearly$indicate$on$these$drawings$the$regulatory$regions$that$would$be$included$in$these$reporter$genes.$$$$$$$$$3.$ Indicate$in$a$diagram,$the$expression$pattern$you$would$expect$for$the$reporter$gene$shown$below.$$$$$ $$4.$
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