Wright BIO 2110 - Rec1_even-skipped Regulation (3 pages)

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Rec1_even-skipped Regulation



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Rec1_even-skipped Regulation

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Pages:
3
School:
Wright State University
Course:
Bio 2110 - Principles of Molecular and Classical Genetics
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BIO 2110 Genetics Name Recitation 1 Regulation of even skipped gene expression Group Members The even skipped eve gene is expressed in seven distinct stripes in D melanogaster embryos Regulation of this expression is modular i e regulatory modules for each stripe are located at different positions in the eve gene see below Structure of the eve gene Positions of regulatory are indicated by stripe number eve expression pattern 1 Explain why these different regulatory modules would cause LacZ to be expressed in different regions of the embryo i e i How do these modules differ from each other and ii How do the different regions of the embryo in which stripes appear differ from each other 2 The diagrams below show LacZ expression patterns obtained from two different eve reporter genes Draw the structures of the reporter genes that would produce these patterns of LacZ expression Clearly indicate on these drawings the regulatory regions that would be included in these reporter genes 3 Indicate in a diagram the expression pattern you would expect for the reporter gene shown below 4 eve expression in the stripe 2 module is regulated by four transcription factors bicoid bcd hunchback hb giant gt and kr ppel kr The expression patterns of these transcription factors are shown to the right In mutant embryos where either bcd or hb are eliminated by mutation eve stripe 2 reporter gene expression is absent from stripe 2 parasegment 3 In gt mutant embryos eve stripe 2 reporter genes are expressed in parasegments 1 2 and 3 In kr mutant embryos eve stripe 2 reporters are expressed in parasegements 3 and 4 Based on these results do each of these transcription factors bcd hb gt and kr act to enhance or silence eve expression through the stripe 2 module


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