Madison McVeyCHEM 237 – 549November 2, 2016Thin Line Chromatography Lab ReportResults: The Anacin pill was assigned to my lab station. By using TLC techniques in a 100% ethyl acetate solution and comparing the Rf values, I was able to determine the pill contained both aspirin and caffeine. The Rf values of each standard were found using the formula below and compiled into the following table: Rf =distancemoved by compounddistance moved by solventCompound Name Rf values in Ethyl Acetate (Distance moved by solvent = 6.8 cm)#1 - Naproxen 0.27#2 - Acetaminophen 0.30#3 - Aspirin 0.38#4 - Caffeine 0.18#5 - Ibuprofen 0.79#6 – Caffeine/Ibuprofen 0.28Anacin 0.10, 0.46Aleeve 0.79Advil 0.33Discussion: In the thin line chromatography lab, students used this lab method to determine the identity of chemicals used in common pain relievers. Each bench was assigned a group and a pill. Thin line chromatography techniques were used to determine the distance each standard and each pain reliever traveled. In the groupsof three, each member performed TLC in a different solution, either, 100% ethyl acetate, 100% hexane, or 75% ethyl acetate/25% hexane. Based on the results from the TLC test in 100% ethyl acetate, where two distinct compounds were present on the chromatogram, it was evident that Anacin contained caffeine and aspirin. This conclusion was made because the Anacin Rf values of the two dots of 0.10 and 0.46 were the most similar to that of the aspirin and caffeine standards of 0.38 and 0.18, respectively. There were no unexpected events that occurred during the experiment. Of course, the results of the Rf values between different groups may slightly differ because of changes in the experiment environment, for example, temperature or because of simple human
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