Experiment 2 Microscopic Slide Examination Skin The skin is a large organ that provides protection to the body In this experiment you will examine a prepared slide of human skin with hair follicles Materials Human Skin Digital Slide Image Procedure 1 Observe each human skin digital slide image 2 Distinguish between the epidermis and the dermis layers Also look for the dermal papillae sweat gland ducts hair follicles and keratinocytes Human Skin 100X 2013 eScience Labs LLC All Rights Reserved Human Skin 400X The stratum corneum contains primarily keratinized cells Dermal papillae can form ridges that aid in gripping fingerprints Human skin 1000X The layers of the epidermis can be distinguished at this magnification The keratinocytes of the stratum granulosum contain dark staining granules 2013 eScience Labs LLC All Rights Reserved Human skin 1000X Enlargement of a hair follicle located in the dermis The kerantinized hair root and the hair matrix region of cell division are enclosed by the hair follicle 2013 eScience Labs LLC All Rights Reserved Post Lab Questions 1 Label the arrows in the following slide image A Epidermin Layer B Sweat Gland Duct C Dermal Papillae D Dermis Layer 2013 eScience Labs LLC All Rights Reserved 2 For the following questions state whether the statement pertains to the epidermis or dermis a b c d e Dermis This layer consists of the papillary layer and the reticular layer Epidermis Composed of keratinized stratified Squamous epithelium Epidermis Langerhans cell and Merkel cell reside in this layer Dermis Composed of dense irregular connective tissue Epidermis The fingerprint pattern unique to each individual is created in this layer f Epidermis Outermost layer of skin g Epidermis This layer has laminated granules and keratohyalin granules within the stratum granulosum h Dermis The dense supply of blood allows this layer to play a part in body temperature regulation 3 List the five layers of the epidermis from most internal to most external and describe their function Stratum basale Contains the only cells that can divide by mitosis Keratin is produced Stratum spinosum Responsible for skin s strength and flexibility Stratum granulosum Produce a lot of keratin in this layer and become flatter and more brittle Stratum lucidum Is made of dead keratinocytes from the granulosum It is clear and transparent It is found on thick hairless parts of our body Stratum corneum Layer of skin we see with our eyes They are dead skin cells filled with keratin It is the hard protective layer against environmental trauma It can repel water This entire cycle takes from 25 45 days 4 List the two layers of the dermis from most internal to most external and describe their function Reticular Layer Strengthens the skin and provides the skin with elasticity It contains hair follicles and sweat glands Papillary Layer Provides the epidermis with nutrients to produce skin cells It also regulates the temperature of the skin and body Also removes cellular waste products that could kill the skin cells 2013 eScience Labs LLC All Rights Reserved
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