Objective:To use Thin Layer Chromatography (TLC) to determine the probable identity of the major active ingredients in commercial analgesic preparationsExperimental Procedure:- You will be working with two other students to complete this lab.- Obtain an analgesic tablet, 3 normal phase TLC sheets, a spotting capillary- Each student in the group selects different analgesic tablets.- Grind tablet with 4 mL of reagent grade methanol- Then gravity filter the suspension through a folded 11cm piece of filter paper in a short stem funnel.- Prepare TLC sheet for spotting- Apply 5 spots to each TLC plate (you will use the same spotting capillary throughout this experiment)- Solutions of 6 standards are available for use- Use templated on procedure sheet to mark your TLC plates before spotting- Spots designated S1-S6 are the 6 standards. Spot A is your analgesic tablet; Sports B & C are the extracts of the tablets of the other two people in your group. (Each student uses a different solvent)- The 3 solvents are hexanes, ethyl acetate, and 75% ethyl acetate-25% hexane.- Pour enough developing solvent into TLC jar (~5 to 6 mm of liquid from bottom of jar)- Place cap on top of the jar and seal it. Let solvent rise until its about 7 to 15 mm from the top of the sheet- Remove the TLC sheet and mark the solvent right away. - After drying, view the TLC under the UV lamp & circle the spots.- Calculate the Rf value of each spot- Compare results with the group and determine which solvent give the best separation of the standards- Each student then uses the 3rd TLC plate with the best solvent to compare your sample with the standards that appear to be in your sample.- Make sure you record Rf values for the standards and the components of your analgesic sample in each solvent systemReagents:- Methanol- Aspirin- Ibuprofen- Acetaminophen- Ketoprofen- Naproxen sodium- Caffeine- Hexane- Ethyl Acetate- 75% ethyl acetate-25% hexaneApparatus:- Spotting capillary- Normal phase TLC sheets- Mortar & Pestle- TLC jar- TLC plate- Gravity Filter- 11 cm piece of filter paper- Short stem funnelSafety:- Dispose of chemicals, TLC spotting capillaries, and contaminated paper in correct containers- The silica gel on the TLC plates can damage eyes - Never look directly into the ultraviolet lamp- DON’T rinse or wash the TLC jar after disposing of the solvents- ALL of the analgesics are considered