MCB 2210 Cell Biology Instructor Dave Dagge david dagge uconn edu Teaching Assistant Sean Stoessel sean stoessel uconn edu O ce Hours Tues Wed Thur 4 5 30pm BPB401 MCB 2210 This course does not require a textbook But these are good references more detailed MCB 2210 This course does not require a textbook But these are good references less detailed MCB 2210 HuskyCT site Lecture slides will be posted on the class web site in pdf format so you can download them These lecture notes will serve as your textbook We will also be posJng lecture audio Problem sets will be posted on the site Exam RegistraJon Links will be posted Various announcements will be posted on the site CHECK IT REGULARLY MCB 2210 Problem Sets There will be two problem sets distributed for each of the 4 exam blocks of the course They will not be graded Some informaJon is taught in the PS quesJons Many most exam quesJons will be taken in modi ed form from these problem sets There will be o ce hours available with the TA to go over quesJons about the problem sets DO THE PROBLEMS understand why the right answer is right and the wrong answers are wrong AND THE EXAMS SHOULD NOT BE A PROBLEM Refer to lecture notes and lecture audio for related topics MCB 2210 Exams There will be four exam dates The rst three will be Block exams occurring on Oct 2 Oct 23 Nov 13 all Fridays The last will include the Block 4 exam and a cumulaJve Final on the scheduled nal exam day Dec 16 You may drop or miss one of the block exams but you may not drop or miss the Final There will be one me prior to the scheduled Jme at which students with a legiJmate con ict may take the exam We may or may not grant this request If you cannot make the early exam you may drop that exam MCB 2210 Exams will be taken at the TesJng Center Rm 110 Arjona On each scheduled Exam day there will be 4 possible Jme periods to take the exam Prior to the Exam you must follow the Exam RegistraJon link on the Husky CT site to sign up for one of the periods They will be lled rst come rst serve Exams will be taken on computer terminals at the TesJng Center You must arrive at 110 Arjona at least 10 minutes before the exam period You must have your UConn ID with you to sign in You may only bring a pen or pencil to the computer terminal and scratch paper will be provided At the start of the Exam Period you will be given a password to access the Exam via the MCB 2210 HuskyCT site The password will allow you access to the Exam only once and the exam must be completed by the end of the Exam period MCB 2210 Syllabus and academic misconduct The syllabus is available on the HuskyCT site It includes the academic code which you are expected to follow Academic misconduct in any form is in violaJon of the University of ConnecJcut Student Conduct Code and will not be tolerated This includes but is not limited to Copying or sharing answers on tests Claiming to have a con ict with the Jme scheduled for the nal if the other exam is not actually occurring during the scheduled Jme Having someone else take tests for you Depending on the act a student could receive an F grade on the test F grade for the course or could be suspended or expelled We take cheaJng very seriously Don t do it How to do well in this class Look over lecture notes before class Listen and understand concepts during lecture Take notes that help you highlight concepts Review or copy over notes aker class while fresh Review the lecture audio Do the problem sets Ask the TA for help Ask other students for help Ask the instructors for help THINK this is not a memorizaJon course it is a concepts course Why should you come to class Students who a end the lectures usually perform be er on the exams than students who do not a end Students who come to class can see where the laser is poinJng and have access to some movies that are no longer available on YouTube Students who come to class can ask and answer quesJons You should be interacJve Syllabus Outline Block 1 FoundaJons Basic organizaJon of cells methods used to study cells and a very brief review on membranes and proteins CommunicaJon across the plasma membrane between the cell and the outside world Block 2 Synthesis and tra cking of proteins to organelles CommunicaJon between the inside of the cell and the plasma membrane Block 3 The cytoskeleton and cell dynamics Block 4 The cell cycle specialized cells and Jssues and cell behaviors Things You Should Hopefully Already Know Something About Chemistry Bonds Covalent ionic hydrogen pH Water Solubility Biological Molecules Nucleic acids Proteins Lipids Sugars The Central Dogma DNA makes RNA makes Protein DNA double helix and replicaJon heredity Genes coding noncoding DNA promoters TranscripJon decoding DNA to make mRNA TranslaJon decoding mRNA to makes proteins Protein Structure and FuncJon Amino acid basics Proteins fold into speci c 3D shapes Enzymes carry out chemical reacJons needed for life Biological Sizes from Wikipedia 0 1 nm Diameter of a hydrogen atom 0 8 nm Amino Acid 2 nm Diameter of a DNA Alpha helix 4 nm Globular Protein 7 nm AcJn lament 10 nm Cell membrane thickness 11 nm Ribosome 25 nm Microtubule 30 nm Small virus Picornaviruses 50 nm Nuclear pore 70 nm Transport vesicle 100 nm HIV 120 nm Large virus In uenza virus 150 250 nm Very large virus Rhabdoviruses Paramyxoviruses 150 250 nm Small bacterium Mycoplasma 200 nm Centriole 200 500 nm Lysosomes 200 500 nm Peroxisomes 1 10 m Most Prokaryotes 1 2 m E coli bacterium 3 m Mitochondrion 6 m Nucleus 9 m Human red blood cell 10 30 m Most Animal cells 10 100 m Most Plant cells 90 m Amoeba 100 m Human Egg 1 mm Diameter of the squid giant nerve cell 120 mm Diameter of an ostrich egg a dinosaur egg was much larger 3 m Length of a nerve cell of gira e s neck Cell Size Cell Shape and Di usion Cell Size Shape Most eukaryoJc cells are 10 30 m in diameter but vary greatly in shape Small molecules move into and out of cells by di usion through the membrane O2 and nutrients must di use into the cell and CO2 and waste products di use out of the cell As a spherical cell gets larger its cytoplasmic volume increases as a cubic funcJon V 4 r3 3 while its membrane surface area increases as a square funcJon 4 r2 The amount of transport is limited by the amount of surface area Surface area cannot keep up with volume This is a limit to the size of metabolically acJve cells Growth is oken coupled with division to maintain cells at …