Order: Diptera Suborder: NematoceraFamily: Culicidae Mosquito mouthparts Palp Length - Long (nearly as long as proboscis) - Short (much shorter than proboscis) Anopheles spp.- ancient genus of mosquitoes- name means “useless” in Greek- long palps- “perpendicular” feeding- malaria vectorsAnopheles quadrimaculatus- Wing scales all darkShort Long- wing with 4 dark spotsAnopheles punctipennis• wing scales pale and dark• two pale spots on front margin of wingProboscis ShapeLong and Strongly CurvedStraight or Slightly CurvedToxorhynchites rutilus septentrionalis• non-bloodfeeding• predatory larvae!Uranotaenia sapphirina• iridescent blue scales on thorax and wingsAbdomen Shape- Pointed vs. blunt Pre-Spiracular Setae - Present vs. absent Long and strongly curved StraightPsorophora spp.• prespiracular setae present• fairly large (but this is not reliable!Psorophora ferox - Probosciso Palps and proboscis dark - Legs o Shaggy, dark and purplish o Tarsal segments 4 and 5 white Psorophora ciliata- Probosciso Palps 1/3 as long as proboscis o Proboscis yellowish dark at the bottom - Legs o White with white basal bands “shaggy” Aedes spp.• prespiracular setae absent• robust• some with clear black and white patternsAedes aegypti• lyre shape• Dengue vector• Yellow Fever vectorAedes albopictus• straight white line• Dengue vector• outcompetes Ae. aegypti• vectors LOTS of things•Aedes vexans• brown abdomenOrthopodomyia signifera• scutum with thin longitudinal lines of white scalesWing Scales- Broad with Pale and Dark Scales vs.Narrow and Mostly DarkCoquillettidia perturbans• pretty, feathery scales• no physical specimenSubcosta of Wing- Tuft of Setae vs. No Tuft of Setae- Culiseta inornata• tuft of setae presentCulex quinquefasciatus• no tuft of setae• should be VERY familiar to you• WNV vectorLarval MosquitoesSiphon Anopheles spp.• siphon absent Basal Pair Branched Ventral Setae Culiseta spp.• siphon with basal pair of branched ventral setae Siphon Setae- 3+ pairs ventralsetae- 1 pair ventralsetae Culex spp.• 3 or more pairs of ventral setaeSaddle- complete rings anal segmentAbsent Present Absent Present 1 pair 3 pairs- incomplete ring OR if complete, not pierced Psorophora spp.• anal segment completely ringed bysaddle• pierced on ventral side by tufts ofventral brushAedes spp.• anal segment not completely ringed by saddle• if complete, not pierced by tufts of ventral brush