ZOL 341 1st edition Lecture 25 Outline of Last Lecture I Mutagenesis II Recombinant DNA III Linear DNA IV Vector V Plasmids VI Bacteriophages Outline of Current Lecture II Transgenes III Eukaryotic expression vectors IV Site directed mutagenesis V Shuttle vector Current Lecture transgenes expression vectors contain the sequences needed for proper expression of the transgenes transgene must not contain introns eukaryotic expression vectors used in yeast or in tissue culture site directed mutagenesis nucleotide changes can be made in a DNA sequence desired change in the middle of primer primer used in PCR nonmutant plasmids selectively degraded shuttle vector can replicate in bacteria or in yeast manipulations in bacteria expression in yeast These notes represent a detailed interpretation of the professor s lecture GradeBuddy is best used as a supplement to your own notes not as a substitute circular vector single cross over event integrates entire plasmid double crossover integrates only a segment linear vector single crossover results in deletion often used to introduce loss of function alleles
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