CPSC 382Organic Chem Ag Biol ProcTopic #7 - Methods Used in the Separation and Structural Analysis of Bio-organic Molecules1. Tissue extraction2. Resolution of compounds3. Solvent partitioninga. two-phase systemsb. partition coefficient4. Chromatographya. definition (mobile phase, non-mobile phase)b. paper chromatographyc. thin-layer chromatography (TLC)d. high-performance liquid chromatography (HPLC)(1) column chromatography principles(2) reverse-phase vs. normal phasee. gas chromatography5. Structure determinationa. general principles of spectroscopyb. UV-Visible spectroscopyc. IR spectroscopyd. nmr spectroscopye. mass spectrometry6. “Metabolomics”Paper chromatography: Thin-layer chromatography:High performance liquid chromatography:Spectroscopy:UV-Visible spectroscopy:IR spectroscopy:NMR spectroscopy:Mass
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