Fundamental Genetics 8813 Exam 3 FORM A Name amen mm We Student number Recitation section number 4jgfi A3 3 0 1 6 5 37 Multiple Choice Section Choose the single best answer for each question Questions 1 15 are worth 5 pts each 1 To map genes of a bacterial strain conjugation must be interrupted at given times Suppose you have Hfr cells of genotype a b 0 d e 8tfs and F cells of genotype a b C d e StIR and you combine these two cultures in liquid medium in four blenders at time 0 After intervals of 3 6 9 and 12 minutes you turn on successive blenders This graph shows the results of plating the cultures of each blender What is the correct order of the genes on this chromosome a 3c 1 a at c C Jl I 3 e e 6 9 M i n u t e s of recombination b 12 5 O3 C36 319 Agabcde acebd C b e c a d 1i s unknown D daceb E becad 2 Which of these haploid strains produces B galactosidase constitutively but does not produce permease 7 1 P 0 2 Y B P 0 2 YK I P 0 2 Y I P 0 2 Y P 0 2 Y3 For a given cross the expected number of double recombinants is 20 and the observed mber of double recombinants is 15 What is the interference calculation I 0 25 B 1 33 E cl C 90 3 20 a D 0 75 O i l 0 7 3 E 15 For numbers 4 and 5 below choose the enzyme production scenario A F that best fits each genotype giv an A indicates that the enzyme is produced in significant amounts a indicates that enzyme production is absent or negligible Each enzyme production scenario may be used once more than once or not at all Assume that glucose is absent in each case A Lactose pr esent beta gal permease lactose absent beta gal permease B Lactose present beta gal permease lactose absent beta gal permease Lactose p esent beta gal permease lactose absent beta gal permease D Lactose p esent beta gal permease lactose absent beta gal permease E Lactose present beta gal permease lactose absent beta gal permease F Lactose present beta gal permease lactose absent beta gal permease 4 Iac lac 7 aco lacZ lacY NC N0 Fr nabf V35 1 U 4 5 lachaEP aco 221agfi ac lacp IacO lacZ EV T T 3 1 759 No 564 at J a m m i e n eed a C 6 In five Hfr trains each of which was used to burld a tIme of entry map the genes entered the recipient cell as follows fl 5 L A C T if Strain1 SLACTF J L l L StrainzzNPlFTCA T c A A r Strain3 TFPNUY Strain4ISHYUNP Strain5 UNPFTC W Which of the following represents a correct compilation of these results 8 LA CT F P N HC U N u y B NPFTSLACHUT TC A L S P N U Y H D UNPFTCALSTF SLACTFPNUYH 7 The synter ic genes A and Z are linked A cross between two parents AAZZ and aazz produces F1 progeny with the AaZz genotype What are the possible arrangements of alleles on the F1 Progehy s chromosomes AZ az Az aZ Aa Zz AZ Az or az aZ El A Z or a z Ag X AZ v52 az fi l m 01A2 Aflzz 8 Which of ti e following could NOT have contributed to the genotype of An XXY individual l l A Nondisjun tion at meiosis ll in the male parentD B Nondisjun 3tion at meiosis in the female parent Wondisjunction at meiosis II in the female parent ondisjun ction at meiosis l in the male parent 1 e Q c 1 100 Use the following data to answer questions 9 11 In the tomato three genes are linked on the same chromosome Tall is dominant to dwarf skin that is smooth is dominant to skin that is fuzzy like a peach and fruit with a normal round shape is dominant to oblate shape A plant that is true breeding for the dominant traits was crossed to a dwarf plant with peachy skin and oblate fruit The F1 plants were then testcrossed The following progeny were obtained in the F2 T 6 0 1 6 d w a r f Number of progeny Phenotype 151 Taltsmooth normat SWVUOM 5 f0 33 Tall smooth oblate 11 Tall peach oblate R 3 rd Md r 033 9 it 5 Tall peach normal 155 Dwarfrp each oblate 29 Dwarf peach normal K via 12 Dwarf smooth normal T5 d Sr Idiff 4 Dwarfrsmoothfibiate 9 Which gene is located in the middle of the other two 3 The genethat codes for height A 9 he genefthat codes for skin texture The geneithat codes for shape r 7 carR 7 k r 10 What is the distance between the height and shape genes A 5 75 B 8 Ts 155 R C 15 5 f D 17 75 E 21 25 5 75 ll i ll t 3 fi t 7204 E l W 2 i l 5 7 7 R I Q00 5 11 Which of the following is a progeny class representing a single crossover between the shape and skin texture genes Dwarf peach oblate B Dwarf peach normal Dwarf smooth normal N T5 5 5r Dwarf smooth oblate 12 You have a DNA sequence of 1600 bp To characterize it you decide to make a restriction map Cutting with the first enzyme you show by electrophoresis that the fragments produced are 400 and 1200 bp respectively After using the second enzyme you recover 700 bp and 900 bp fragments After treatment with both enzymes fragments were 300 400 and 900 bp Which is the correct map A 900 J 4oo 3 B 400 9op 3oo C 300 400 900 Q 400 300 900 400 1001 3oo 900 I W a 400 r m i300 A R 700 waft i k i 00 3 x 2 OIOO C100 M l 13 For a given cross the expected number of double recombinants is 20 and the observed number of dc uble recombinants is 15 What is the coefficient of coincidence c A 0 25 B 1 33 l A 9 2 20 f xc 93 75 15 4s 14 The folio Ming map shows four deletions 1 to 4 1 2 C 9 Ccle 3 4 L Five point mutations a e in the region are tested against these four deletion mutants for their ability or inability to give Wild type recombinants with the following results a b c d e 1 2 3 4 hat is the order of the point mutations cedba a I 3 C l B dbaced B dbeca abdec b I 5 d 3 3 e 2k 15 For the fc llowing gene Mspl and Hpall restriction sites and their positions are indicated here Hpall ismethylgflon sensitive while W o t That is if an Hpall restriction sequence is methylated …