Mentoring Worksheet 10 Gene Regulation Learning Goals Can you Explain how changes in gene expression enable cells to respond to changes in their environment Draw a diagram showing the similarities and differences between eukaryotic and prokaryotic genes Predict whether a gene such as trp or lac operons would be actively transcribed if given a description of this gene s regulatory region and a description of conditions experienced by the cell containing that gene Terms and Concepts housekeeping inducible genes inducible repressible promoter cAMP CRP positive negative regulation transcription factors inducer repressor operon trp lac operons coinducer corepressor operator enhancer Mentoring Worksheet 10 Gene Regulation Section Please circle 9 AM 11 15 AM Group Group Members present Please turn one copy of this completed worksheet into your mentor at the end of the session 1 The Trp and Lac operons are found in E coli and display regulated patters of expression a Distinguish between positive and negative regulation of gene expression positve the protein is activator negative the protein is the repressor b Use a diagram to explain whether the trp and lac operons display positive and or negative regulation promotor operator RNA polymerase promotor operator activate repressor Text Cmap low glucose CAP Mentoring Worksheet 10 Gene Regulation 2 You perform an experiment looking at gene expression in E coli collected from the intestines of several volunteers a short while after they have had a meal Explain whether you would expect to see the Trp and Lac operons being expressed in bacteria isolated from each of the following people a Bob after a ham sandwich with a regular soda i Lac Operon off ii Tryp Operon off since try present so it turn is off b Susan after a salad with turkey cheese no dressing and a glass of lowRfat milk i Lac Operon on ii Tryp Operon off same reason as one c Jane after an egg and cheese omelet and a diet soda i Lac Operon on cuz cheese like milk ii Tryp Operon off d Tom after spaghetti with tomato sauce bread and wine i Lac Operon off ii Tryp Operon on