DOC PREVIEW
CU-Boulder MCDB 2150 - Transgenic Organisms

This preview shows page 1 out of 4 pages.

Save
View full document
View full document
Premium Document
Do you want full access? Go Premium and unlock all 4 pages.
Access to all documents
Download any document
Ad free experience
Premium Document
Do you want full access? Go Premium and unlock all 4 pages.
Access to all documents
Download any document
Ad free experience

Unformatted text preview:

MCDB 2150 1st Edition Lecture 21Outline of Last Lecture - Recombinant DNA technologyo How to clone a geneOutline of Current Lecture - Transgenic organismso Transgenes- The “knockout” mouseThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.Biotechnology – Transgenic animalscDNA = only contains the genes that are expressed in an organism or tissuecomes from a collection of all mature mRNAs, then reverse transcriptase is used to create the cDNA library (without non-coding regions)Transgenic organisms = contain recombinant DNA in their genomeTransgene = a cloned gene that is transferred from one species to another- ^^must be integrated into a chromosome to become stable- integration:o recombination techniqueso random insertion into a “nick” in the DNAIntroducing transgenes:- gene replacement: cloned gene replaces chromosomal copy by homologous recombination- gene knockout: chromosomal copy replaced with inactive mutant- gene addition: cloned gene integrates at a different site; both copies (original & transgene) are present- Must be done at an early embryonic stage so all cells have recombinant DNAThe transgenic “knockout mouse”- Model for human disease (study of homologs – evolutionarily similar genes)- Most useful for diseases caused by a single gene- 1 – introduce a non-functional copy of the gene by homologous recombination (only occurs in regions where the sequence is similar)o RARE – selection is criticalo Often use NeoR gene (where non-functional gene goes) because this is an anti-toxin gene Grow the animals in the presence of the toxinso BUT – could be random integration ratherthan homologous recom. SOOO…. Use a TK gene as a negative selector (organisms with this gene cannot grow in the presence of gancyclovir) If homologous recombination = only the TK gene or the NeoR gets inserted (not usually


View Full Document
Download Transgenic Organisms
Our administrator received your request to download this document. We will send you the file to your email shortly.
Loading Unlocking...
Login

Join to view Transgenic Organisms and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Transgenic Organisms 2 2 and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?