Bio 201 1st Edition Lecture 9Outline of Last Lecture I. Organelle FunctionA. CentrifugationII. The OrganellesA. The NucleusB. The Endomembrane SystemIII. George Emil PaladeOutline of Current Lecture I. Endomembrane System ContinuedA. Pulse- Chase ResultsB. SecretionC. EndocytosisCurrent LectureI. Endomembrane System contd.A. Pulse- Chase results- 1-3 minute chase silver grains mostly in RER. 10-20 minutes chase silver grains in RER and golgi. 120+minutes chase silver grains in secretory granules. Without chase the radioactive amino acids are present throughout and with chase radioactive amino acids are removed after 3 minutes.B. Secretion- Pathway: RER cis-golgi medial-golgi trans-golgi secretory vesicle plasma membrane/secretion-Constitutive- secretion that is constant and slow and steady. Fusion is energetically favorable and happens spontaneously in this pathway. -Regulated- secretion where vesicles are stored at plasma membrane until stimulated by a signal (often calcium). AKA exocytosis. C. Endocytosis- the process by which material is taken into the cell membrane from outside the cell. 3 types:1. Phagocytosis- endocytosis of larger, solid matter, generally for nutrition or pathogen removal. These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.2. Pinocytosis- endocytosis of membrane and small, soluble contents only. 3. Receptor-mediated endocytosis- internalization of small vesicles in response to signaling molecule binding to a receptor on the plasma membrane. This often involves clathrin. -Lysosomes digest/degrade endosome contents using low pH and pH-dependent enzymes. After endocytosis digestion
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