On-line quiz 5Q.1 Which of the following statements is true?a. One codon can code for two different amino acids.b. tRNAs carry the codons.c. mRNAs have anticodons.*d. One amino acids may have more than one codonQ.2 Translation is a process that:a. Converts DNA sequence into RNA sequence.b. Polymerizes RNA bases into RNA.*c. Synthesizes polypeptide following codon order in the mRNAs.d. Synthesizes polypeptide following anticodons in the tRNAs Q.3 What would be the amino acid sequence if the following mRNA is translated starting at the 5’ most base: 5’-GCGAGUGCAUAG-3’?*a. Ala-Ser-Ala-stop.b. Ala-Met-Ala-stop.c. Met-Glu-Gly-Tyr.d. Met-Thr-Pro-stop.Q.4 Polynucleotide phosphorylase is an enzyme that synthesizes:a. DNA using an RNA primerb. RNA using an RNA primer.*c. RNA without a primer.d. RNA using dNDPs as substrates.Q.5 If you synthesize RNA in vitro using polynucleotide phosphorylase, ADP, CDP and GDP, how many different triplet codons are expected in the population of RNA synthesized?a. 8.b. 64.c. 9.*d. 27.Q.6 Suppose you synthesized RNA in vitro using ADP and UDP in proportion 3:1, and polynucleotide phosphorylase. What would be the expected frequency of a stop codon in the RNA population?a. 1/64.*b. 9/64.c. 3/64.d. 0.Q.7 Suppose you synthesized RNA in vitro using ADP and UDP in proportion 3:1, and polynucleotide phosphorylase. What would be the expected frequency of a isoleucine (ile) codon in the RNA population?*a. 12/64.b. 3/64.c. 9/64.d. 1/64.Q.8 Suppose an mRNA codes for a polypeptide made of 200 amino acids. If the 100th codon is removed from this mRNA, which of the following results is expected?a. A frame-shift mutation after the removed codon.b. A frame-shift mutation before the removed codon.*c. No change in the amino acid sequence after the removed codon.d. The mutated mRNA will code only for the first 99 amino acids.Q.9 The sequence of the anticodon in the tryptophan (trp) tRNA is:a. 5’-ACC-3’*b. 5’-CCA-3’c. 5’-CCI-3’d. 5’-ICC-3’Q.10 How many sense codons are found in eukaryotes and prokaryotes?a. 64.b. 50.c. 30.*d.
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