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TAMU GENE 412 - Recombination Frequency

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GENE 412 1st Edition Lecture 4 Outline of Last Lecture I. X2 Chi-Squared TestII. P < .05 Concludes?Outline of Current Lecture I. Gametic Phase (Linkage Equilibrium)II. Things Population geneticists likeCurrent LectureI. Gametic Phasea. Two Locus H.W.i. CAT CAT CAT CAT CAT CAT CAT CATGAT CAT CAT CAALast row shows Two Locus H.W. because recombination or crossover must occur to get back to normal genotypeb. Genetic Polymorphism i. Presence of 2 or more alleles at 5% or 1%1. 1% recombination = 1*106 nucleotides2. recombination does not happen too often when genes are close togetherAB abAB AB/AB AB/abab AB/ab ab/abc. Ab/aB – recombination is only way to get this genotype i. AB/ab = Couplingii. Ab/aB = Replusiond. D is the percentage of recombination i. D = f(AB)*f(ab)-f(Ab)*f(aB)ii. If D > 0 then Dmax is lesser of f(Ab) or f(aB)iii. If D < 0 then Dmax is lesser of f(AB) or f(ab)These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.iv. D’ = D/Dmaxv. Best marker for recombination is the largest D’II. Things population geneticists likea. Like lots of alleles because that means a lot of information b. Codominant or incomplete dominantc. Like to indentify alleles with confidence; don’t confuse two genes with two alleles of one gened. Ways to look at datai. Microsatellite Analysis1. LOOKING FOR MOBILITY2. Different copy number can give you a different number of alleles ii. Mitochondrial DNA analysis 1. Inherited mother to opffspring2. Take amplified piece with restriction enzyme to determine if DNA has a particular sequence; will cut DNA if sequence is present iii. Randomly Amplified Sequences of DNA (RAPID’s)1. Get amplified product, but can’t tell if two products alleles or two completely unrelated alleles (PROFESSOR SAYS HE DOES NOT LIKE


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