BCH 380 1st Edition Lecture 8 Outline of Last Lecture I. Gene transcription Outline of Current Lecture 1. RNAA. mRNA, rRNA, tRNA 2. Message RNA processinga.5’ cap, 3’ poly-A tail, RNA splicingCurrent LectureImportant RNAs:- rRNA- mRNA = only one that can make proteins - tRNAMessage RNA processing (only in eukaryotes):- 5’ cap- 3’ poly-A tail- RNA splicing5’ CapLocation: nucleusPlace: 5’ position of RNATime: Rapidly after initiation of transcription What: 1. Addition of one rGTP at the 5’ end2. 4 methyl groups Function: 1. Translation site recognition 2. Stabilize RNA3’ Poly-A TailLocation: nucleusPlace: 3’ position of RNATime: after cleavageWhat: Adding 50 to 250 AFunction: stabilize RNARNA Splicing - Forms mature RNA5’ consensus AG/GUAAGU [middle part is removed] CAG/G 3’ consensusPre-mRNA RNA splicing
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