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CORNELL BIOMG 1350 - Microscopy and Organelles

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BIOMG 1350 1st Edition Lecture 1Outline of Last Lecture I. This is the first lecture!Outline of Current Lecture II. Microscopya. IMFs, GFPsb. Light vs. Electron MicroscopyIII. OrganellesCurrent LectureII. General Microscopy- Resolving power: ability to distinguish two close objects- Maximum resolving power depends on wavelength of the illumination.A. IMFs, GFPsa. IMF: Indirect Immunofluorescence.i. Sensitivity: Fluorescence- absorbs wavelengths and emits a different, longerwavelength.ii. Specificity: Antibodiesiii. Fix cells and make them permeable to antibodies 1. Primary antibodies vs. secondary antibodiesb. GFP: Green Fluorescent Protein i. Sensitivity: GFP fluorescence. (Shine blue light, glow green light)ii. Specificity: GFP protein fusion (fuse gene of protein of interest and GFP gene)1. Ex. Plasma membrane protein and GFPB. Light vs. Electron Microscopy:a. Light: smaller, objects blue, 200nm minimumb. Electron: better resolving power thank light microscope, 2nm minimum, hard to label specific structuresc. To see cells- need to use electron microscopeIII. Organellesa. Parietal Cell- pumps acid into stomachb. Size of an average organelle: 1-2 micrometersc. Prokaryotes: bacteria, archea, no nucleus d. Eukaryotes: nucleus, communication through nuclear pores, plasma membranes encloses membranese. Mitochondria: double membrane, endosymbiosisf. Chloroplasts: double membrane, convert light into sugars.These notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.g. Endoplasmic reticulum: continuous with outer membrane of nucleus. Proteins package and sent to Golgih. Golgi Apparatus: sort and process proteinsi. Cytosol: where most biochemical reactions occur, part of the cell that is not membrane boundj. Why do most organelles have internal membranes?i. They allow more membrane surface per cell volumeii. More reactions can be carried outiii. Compartmentalization of functionsk. Origins of some organelles: endosymbiosis (Eukaryote cell engulfed bacterium, these organelles have their own DNA, make their own


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