Biochem review session- 9/3Possible test questions:1. What properties of proteins or peptides are derived from these procedures or determine the behavior:a. SDS- gelb. Isoelectric focusing- separating molecules by differences in their isoelectric pointsc. Gel chromatographyd. Ion- exchange chromatographye. Reverse- phase high performance liquid chromatography (RD-HPLC)2. What is the effect of urea or guanidine HCl on proteins? How was this important in the Antinsen’s experiments on RNAse A?a. Abolish tertiary and secondary structures, destroy them3. Match: (20 points)a. Edman degredationb. RP-HPLCc. Gel chromatographyd. Ion exchange chromatographye. PAGE- polyacrylamide gel electrophoresisf. SDS- gel electrophoresisg. Trypsin digestioni. Criterion of purity __c,d,e____ii. Size separation __c,f___iii. Subunit molecular weight _f____iv. PITC __a___v. Concentration _____vi. Separation by net charge __d___vii. Peptide separation __b___viii. Dialysis _____4. On gel filtration of the following proteins, in what order will they probably elute from the column?a. RNAse A (M=13 KD)3rdb. Carbonic anhydrase (M=29 kD)2ndc. Bovine serum albumin (M=66 kD)1st5. On electrophoresis on an SDS-gel, after all disulfides are reduced, in what order will these proteins, all single chains, move?a. Larger proteins move slower- depends on molecular weight- smaller protein moves fastest- on gel sheet- a moves fastest6. Given the polypeptide: ala-phe-glu-lys/thr-gly-cys-trp-ala-pro-met-ala-glya. Digest with:i. Trypsinii. Chymotrypsiniii. V8 proteaseiv. CNBr1. Indicate the cleavage site with vertical arrows and the appropriate letter (in pictures)7. Fill in the correct letters (20 points)a. Mb _ii__ _iv__ __v__ ___ ___ ___ ___b. Hb _i__ _iii__ _v__ __vi_ ____ ___ ___i. O2 carrier in bloodii. O2 carrier in muscleiii. Alpha2 beta2 subunit structureiv. Single polypeptide chinv. Heme prosthetic groupvi. Cooperative binding of O2vii. Allosteric control by H+, CO2, 2,3BPGviii. First x-my structureix. M=18