TAMU GENE 301 - Exam 3 Study Guide (4 pages)

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Exam 3 Study Guide



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Exam 3 Study Guide

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Practice questions and vocal for the course. Could be printed and used as the note sheet he allows us to take in.


Pages:
4
Type:
Study Guide
School:
Texas A&M University
Course:
Gene 301 - Comprehensive Genetics
Comprehensive Genetics Documents

Unformatted text preview:

GENE 301 1st Edition Exam 3 Study Guide Lectures Definitions Recombinant DNA Bacterial plasmid DNA joined to human DNA Taq Enzyme similar to E coli DNA polymerase I but capable of withstanding boiling temperatures Enzyme used as a DNA polymerase in the PCR technique Photolyase One type of DNA repair of thymine dimmers that requires no DNA synthesis enzyme FISH Method used to determine the location of a DNA segment on chromosomes A fluorescent dye labeled primer that is known to hybridize to human DNA at only one site in the genome is used to prime human DNA in chromosomes on microscope slide for the PCR reaction A four base pair sequence GACA is used as an unlabeled return primer for this PCR reaction After 17 thermal cycles the slide is observed with a fluorescence microscope What is this process called Dideoxythymidine Compound used in preparing a sequencing gel only for the DNA run in the T lane Southern blot Technique used to transfer the DNA from an electrophoretic gel to a nitrocellulose membrane Walking DNA Process that uses segments of one DNA library to identify adjacent segments in another DNA library Nitro cellulose Absorbs the single stranded DNA eluted from a DNA electrophoresis gel in a Southern Blot RNA polymerase Enzyme that binds to the promoter site 2 3 dideoxyadenosine Used to terminate synthesis in one column of the 4 columns of a DNA sequencing gel Polymerase III Enzyme modified by recA so that it can function as a polymerase in SOS repair Translation Silencing or interfering RNA stops this processes hnRNA Enzyme used to amplify the effects of interfering RNA siRNA Used to stop translation of a specific message in eukaryotes The translation of a message can be halted and the message marked for degradation by which of the following siRNA In eukaryotes which of the following can serve to stop translation AND prevent further translation of a messenger RNA siRNA The polymerase chain reaction was run on whole human DNA using 16 different 8 base pair



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