SC CHEM 321 - UV-Vis Absorption Spectroscopy of Dyes and Caffeine in Beverages (3 pages)

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UV-Vis Absorption Spectroscopy of Dyes and Caffeine in Beverages



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UV-Vis Absorption Spectroscopy of Dyes and Caffeine in Beverages

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Pages:
3
School:
University Of South Carolina-Columbia
Course:
Chem 321 - Quantitative Analysis

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Cameron Kahn Muditha Dias Experiment 7 Postlab 9 April 2014 Experiment 7 UV Vis Absorption Spectroscopy of Dyes and Caffeine in Beverages Data Blank Wavelength Absorbance nm Blank for caffeine 0 088926509 595 0473633 Absorbance 0 0664 505 0133972 0 094130643 635 0545654 0 086760983 500 007843 0 095645539 Sample analyte Wavelength Absorbance Concentration Absorptivity M 1cm 1 625 0570068 0 087645605 nm M 430 018219 0 114484869 Blue standard 595 0474 0 976036 5 045E 06 175832 7056 630 0592651 0 086956121 Red standard 505 0134 0 918359 4 029E 05 20457 40879 Blue Gatorade 635 0546 0 201167 6 507E 07 175832 7056 Red Gatorade 500 0078 0 754319 3 220E 05 20457 40879 Caffeine standard 625 057 0 095704 1 03E 03 28 45 Caffeine drink 430 0182 0 082526 5 668E 04 28 45 Koolaid Blue 630 0593 0 453058 2 082E 06 175832 7056 Koolaid Red 505 0134 0 617031 2 556E 05 20457 40879 the blank must be subtracted from the absorbance to calculate concentration Calculations 2mL of red dye was diluted to 100mL 2mL of blue dye was diluted to 500mL Caffeine was diluted to 5mL A b c Concentration of blue and red standards 1mg 1 mol 2 mL 5 045e 6 M Blue 1 mL 792 86 g 500 mL 1mg 1 mol 2 mL 4 029e 5 M Red 1 mL 496 43 g 100 mL Kahn 1 Absorptivity of blue and red standards A 976036 08896 175832 7056 M 1cm 1 Blue B C 5 045e 6 A 918358 09413 20457 40879 M 1cm 1 Red B C 4 029e 5 Concentration of blue and red Gatorade A 201167 08676 c 6 507e 7 M Blue b 175832 7056 A 754319 0 09565 c 3 220e 5 M Red b 20457 40879 Concentration of blue and red Kool Aid A 0 453058 0 086956 c 2 082e 6 M Blue b 175832 7056 A 0 617031 0 094130 c 2 446e 5 M Red b 20457 40879 Concentration of Caffeine standard 1mg 1 mol 1 mL 1 03e 3 M 1 mL 194 19 g 5 mL Absorptivity of standard A 082526 0664 5 668e 4 M 1cm 1 b c 28 45 1d No we did not completely separate the dyes for red and blue because the wavelength of the highest absorbance for the standards did not match the wavelengths of the highest absorbance for the samples for both red



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