SC CHEM 321 - Design an Analysis: Choosing a Solvent (5 pages)

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Design an Analysis: Choosing a Solvent



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Design an Analysis: Choosing a Solvent

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5
School:
University Of South Carolina-Columbia
Course:
Chem 321 - Quantitative Analysis
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Cameron Kahn Muditha Dias Experiment 6 Post Lab 2 April 2014 Experiment 6 Design an Analysis Choosing a Solvent Data Standard sample Retention time Retention Linalool 54 1mg Compound time min Carvone 51 3mg Carvone 8 851 Eugenol 66 4mg Cinnamaldehyd Cinnamaldehyd 68 6mg e 9 061 eLinalool 7 668 Eugenol 9 716 Hexane 1 Hexane 2 Hexane 3 Hexane 4 Hexane 5 Peak 1 2 3 4 Peak 1 2 Peak 1 2 Peak 1 2 3 4 Peak 1 2 Retention time min 8 4 9 845 11 099 12 216 Retention time min 11 092 12 209 Retention time min 11 092 12 208 Retention time min 3 561 9 84 11 092 12 209 Retention time min 3 565 9 841 Area 33781665 94702316 129836621 146256402 Area 2877097 3170599 Area 2140479 2171796 Area 1681468 384823 1194871 925022 Area 1338244 258383 Diethyl 1 Diethyl 2 Diethyl 3 Diethyl 4 Diethyl 5 Ext Hexane 3 4 Peak 1 2 3 4 5 6 7 8 Peak 1 2 3 4 5 Peak 1 2 3 Peak 1 2 Peak 1 2 Peak 1 2 3 4 5 6 7 8 11 092 12 209 Retention time min 5 332 7 599 8 87 9 841 10 405 10 669 11 093 12 21 Retention time min 5 328 5 398 10 422 11 093 12 21 Retention time min 5 327 10 421 11 093 Retention time min 5 33 10 43 Retention time min 5 33 10 438 Retention time min 3 566 6 553 6 583 7 004 7 127 7 602 7 883 8 858 1028009 698198 Area 5516345 513446 331427 703932 2527230 184887 531566 252097 Area 2212687 2053424 1997559 519374 297530 Area 4205131 1718774 260326 Area 3915410 1598613 Area 3526238 1143825 Area 1490030 1691917 1987969 1876755 1763755 284326332 4099894 158796227 Ext Diethyl 9 Peak 1 2 3 4 5 6 7 Standards 9 081 Retention time min 6 58 7 004 7 141 7 594 7 887 8 853 10 419 2053379 Area 1659910 901954 742805 177660621 1924850 92799763 2672162 Hexane as solvent concentration Retention area x y linalool 0 006492 0 00541 0 004328 0 003246 0 002164 Carvone 0 006156 33781665 0 00513 0 004104 0 003078 0 002052 Eugenol 1 0 007968 94702316 2 0 00664 3 0 005312 4 0 003984 384823 5 0 002656 258383 Cinnamaldehyd 0 008232 e 0 00686 0 005488 0 004166 0 00274 Diethyl Ether as solvent concentration Retention area y x 0 006492 no retention areas were comparable 0 00541 to the standard 0 004328 sample retention 0 003246 table If I had 0 002164 retention areas 0 006156 then I would be 0 00513 able to graph 0 004104 concentration versus area to get 0 003078 a linear function 0 002052 From there I 0 007968 would have been 0 00664 able to solve for x 0 005312 which is the 0 003984 concentration of 0 002656 the analyte 0 008232 0 00686 0 005488 0 004166 0 00274 No graph could be created for Linalool or Cinnamaldehyde because retention areas were not comparable to the table of standard samples No graphs could be created for the standards using diethyl ether as a solvent If atleast 3 retention areas were given for the standards in each solvent then I would be able to create graphs that related the concentration in ppm versus retention area and from there found a line of regression to show the linear relationship I could only graph Eugenol in Hexane 1 Compare the concentrations of the standard analytes vs the extracted analytes for hexane Remember you have 4 analytes Calibration curve for Eugenol in Hexane 12 10 Peak Area 8 6 4 2 0 Ext Hexan e 0 0 0 f x R 0 0 0 01 0 01 0 01 0 01 0 01 concentration ppm Peak Retentio Area n time min 1 3 566 1490030 2 6 553 1691917 3 6 583 1987969 4 7 004 1876755 5 7 127 1763755 6 7 602 284326332 7 7 883 4099894 8 8 858 158796227 9 9 081 2053379 Closest retention area in the hexane extraction for Eugenol is 2053379 Plugging in that number in for y in the linear regression equation will yield x X is our concentration of analyte in the hexane extraction X 0 0031ppm Comparing the 0 0031ppm concentration to Eugenol s set of standard concentration 2 Compare the concentrations of the standard analytes vs the extracted analytes for diethylether Remember you have 4 analytes No graphs could be created due to lack of comparable retention areas for the standards therefore we could not compared any value to the diethyl extraction If I had retention areas available I would do the same steps as stated previously in question 1 where I would use the regression equation to solve for x and then compare that concentration with the set standard concentrations 3 Compare the extraction efficiency of each organic solvent for all analytes Remember you still have 4 analytes This is not practical because each group used different analytes and weighed different amounts of analyte to make their standard set of concentrations for each analyte Getting each groups data would be a hard task to undergo But lets say if everyone used the same analytes and the same masses one could perform a t test comparing our extraction concentrations for each analyte to their extraction concentration for each analyte in the two solvents at the 95 confidence interval to see if the values are statistically different or statistically the same From there we could either accept or reject the null hypothesis If the null hypothesis was rejected for the data not being significantly the same one would have to accept the alternate hypothesis that would state the data was significantly different at the 95 confidence interval The hardest part of the lab was not getting retention areas for each of the analyte 4 Make a recommendation regarding which solvent is better in this context and why include numerical rationale Looking at the class data I would recommend hexane as the better solvent because it yielded more retention times for everyone than diethyl ether To compare the solvents using numerical data one would need us to perform t tests but we did not perform the t tests to get numerical values to support my recommendation


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