SC CHEM 321 - Dilution, Calibration and Error Analysis (8 pages)

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Dilution, Calibration and Error Analysis



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Dilution, Calibration and Error Analysis

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Pages:
8
School:
University Of South Carolina-Columbia
Course:
Chem 321 - Quantitative Analysis

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Chemistry 321L 02M Post Lab Experiment 1 Dilution Calibration and Error Analysis By Cameron Kahn 12 February 2014 TA Muditha Dias Kahn 1 P a g e Single investigator tasks 1 Flow chart depicting dilution strategies Parallel Dilution Strategy 1 30 sucrose written on working bottle Added 500 L of known sucrose Added 1 000 L of known sucrose Added 1 500 L of known sucrose Diluted to 10mL Diluted to 10mL Diluted to 10mL Mass of solution 10 0519g Mass of solution 10 0826g Mass of solution 10 1137g Calculated density 1 005g mL Added 2 000 L of known sucrose Diluted to 10mL Mass of solution 10 202g Calculated density 1 020g mL Kahn 2 P a g e Calculated density 1 008g mL Calculated density 1 011g mL The temperature during dilutions was 24oC Added 2 500 L of known sucrose Diluted to 10mL Mass of solution 10 236g Calculated density 1 0236g mL Parallel dilutions use multiple dilutions to determine the relationship between sucrose and density Strategy 1 involved 5 steps for each dilution Tare volumetric flask add known volume via Eppendorf dilute to the mark 10mL determine the mass of the solution and calculate density Serial Dilution 30 sucrose written on working bottle We took the most concentrated calibrator and label it SD1 From parallel dilutions SD1 has a density 1 0236g The temperature during dilutions was 24oC Added 2000 L from calibrator to volumetric flask Diluted to 10mL This is now labeled SD2 and has a density 0 9946g mL This process continues by taking the newly labeled calibrator previously made add 2 000 Lfrom previous calibrator into volumetric flask dilute to 10mL and then re label new calibrator concentration SD3 SD4 and SD5 SD3 density 0 9939g mL SD4 Density 0 9875g mL Kahn 3 P a g e SD5 Density 0 9825g mL 2 Tabulated data sets Calibration of Eppendorf 1 10 to 100 L 10 standard deviation 25 standard deviation 50 standard deviation 75 standard deviation 100 standard deviation Calibration of Eppendorf 2 100 to 1 000 L 100 standard deviation 250 standard



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