SC BIOL 101 - Catalase Enzyme Activity (8 pages)

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Catalase Enzyme Activity



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Catalase Enzyme Activity

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Pages:
8
School:
University Of South Carolina-Columbia
Course:
Biol 101 - Biological Principles I
Biological Principles I Documents

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Bio 101L Experiment 3 Catalase Enzyme Activity Report Author Cameron G Kahn Report Submitted 8 October 2014 Data Collected 17 September 2014 Laboratory Partners Nilesh Syam Taylor Hollingsworth Teaching Assistant Amanda Havighorst Author s Affiliation Department of Chemistry and Biochemistry University of South Carolina Columbia SC 29208 I pledge that my work meets the standard of the USC Honor Code ABSTRACT This experiment was performed to show the importance of enzyme activity by examining catalase in the decomposition of hydrogen peroxide Enzymes are important catalysis for chemical reactions that happen within living organisms By studying the effects of different concentrations of substrate and environmental changes to the enzyme the rate of reaction can be evaluated and thus allowing one to create the most ideal situation that will make an enzyme the most efficient in catalyzing a certain chemical reaction The results from performing this lab shows the fastest rate of reaction when the catalase is at a warm temperature and hydrogen peroxide at higher concentrations to a certain extent Upon Statistical analysis of both data sets collected only the results from 0 40 0 20 and 0 10 substrate concentration lied within the 95 confidence interval All other results were affected by potential systematic errors thus causing the data to lie outside the 95 confidence interval 1 INTRODUCTION Within the human body many chemical reactions take place in order for our bodies to function Enzymes are protein catalysts which help in speeding up the rate of a reaction 1 One of the more important enzymes in our bodies is a substance called catalase which strictly deals with the breakdown of hydrogen peroxide H 2O2 into water H2O and oxygen O2 The chemical reaction of the decomposition of hydrogen peroxide with catalase as the catalyst can be seen below 2 H 2 O2 2 H 2 O O2 2 Hydrogen peroxide is considered to be cytotoxic meaning toxic to living cells and therefore it is necessary to



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