DOC PREVIEW
MIT 7 013 - Study Notes

This preview shows page 1 out of 2 pages.

Save
View full document
View full document
Premium Document
Do you want full access? Go Premium and unlock all 2 pages.
Access to all documents
Download any document
Ad free experience
Premium Document
Do you want full access? Go Premium and unlock all 2 pages.
Access to all documents
Download any document
Ad free experience

Unformatted text preview:

MIT Department of Biology 7.013: Introductory Biology - Spring 2005 Instructors: Professor Hazel Sive, Professor Tyler Jacks, Dr. Claudette Gardel 7.013 Section Problem-Immunology IA. You would like to generate antibodies against a specific antigen, antigen A, derived from a mammalian virus. You inject a rabbit with antigen A to hopefully elicit antibodies, and you assay antigen A specific antibody levels in rabbit blood every seven days. On day 28 you inject the same rabbit with more antigen A and measure antibody response for the next four weeks. The results of your measurements are shown below. As a necessary control, you tested the blood of this rabbit prior to any injections (0 time point). Days after injectionPresence of antibodies against antigen A (before injection) 0 -7 ++ 14 ++ 21 + 28 + 35 ++++++++ 42 +++++++ 49 ++++++ a) Why is the antibody response low on day 28 and high after day 35? b) Antibodies to antigen A were detected on day 0 (prior to injection of antigen A) in a second rabbit in this study. How could this be explained? c) Suppose on day 28 the rabbit was injected with both antigen A and a different antigen, antigen B. i) Would you expect the levels of antibody against antigen B on day 35 to be higher, lower, or the same as the levels of antibodies against antigen A? ii) Draw a graph indicating the levels of antibodies to antigens A and B vs. time. DayB. A cell clone represents identical cells that have descended from a single progenitor cell. You are a new UROP in an immunology lab and have been given the following task. You are to determine the specificity of two helper T cell clones (THA, and THB) that were found in the freezer. All THA cells express the same T cell receptor (TCR). All THB cells express the same TCR (a TCR distinct from the TCR expressed by THA). You grow two different cell types, a B cell clone or macrophages. To each cell type you add a TH cell clone with either virus 1 or virus 2 and determine if the cells are stimulated to divide. The data is given below. Stimulation is denoted +, no stimulation is denoted -. Cell Type virus added THA THB B cell clone 1 + -B cell clone 2 --macrophage 1 + -macrophage 2 -+ a) Which virus do THA cells recognize? b) Which virus do THB cells recognize? c) Why are THA cells stimulated to grow by B cells plus virus 1, but not by B cells plus virus 2? d) Why are THB cells stimulated to grow by macrophages plus virus 2, but not by B cells plus virus


View Full Document

MIT 7 013 - Study Notes

Documents in this Course
Exam 1

Exam 1

12 pages

Life

Life

4 pages

Cloning

Cloning

2 pages

Load more
Download Study Notes
Our administrator received your request to download this document. We will send you the file to your email shortly.
Loading Unlocking...
Login

Join to view Study Notes and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Study Notes 2 2 and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?