Membranes in BiologyCells and Their MembranesSlide 3LipidsLipid Bilayers (In Vitro)Membranes In VivoThe Complexity of Real-World MembranesThe Motility of Proteins in MembranesMembrane Deformation in BiologySlide 10Quantitative Tools for Tomography: Viral Budding as an ExampleVesicle TransportVesicle Transport: Clathrin CoatingDynamin and Vesicle FissionIon Channels and Transient PermeabilityPhysics of Viral BuddingMembranes in BiologyMembranes in BiologyRob PhillipsCalifornia Institute of Technology(McMahon)Cells and Their MembranesCells and Their MembranesCells and Their MembranesCells and Their MembranesLipidsLipidsLipid Bilayers (In Vitro)Lipid Bilayers (In Vitro)Hydrophobic tails and polar head groups.Favorable for lipids to spontaneously assemble to form bilayers. Molecular Continuum(Avanti Polar Lipids)Membranes In VivoReal biological membranes contain many different lipids & transmembrane proteins!The Complexity of Real-World The Complexity of Real-World MembranesMembranesThe Motility of Proteins in The Motility of Proteins in MembranesMembranesMembrane Deformation in BiologyMembrane Deformation in Biology(McMahon)Membrane Deformation in BiologyMembrane Deformation in Biology(McMahon)Quantitative Tools for Tomography: Quantitative Tools for Tomography: Viral Budding as an ExampleViral Budding as an ExampleTomography producing wide range of images of deformed membrane systems.Calculated Deformation of VesicleDue to Actin Polymerization(Medalia et al.)Observed Membrane DeformationDuring Cell Movement(Dooher et al.)HIV BuddingVesicle TransportVesicle TransportVesicle Transport: Clathrin CoatingVesicle Transport: Clathrin CoatingDynamin and Vesicle FissionDynamin and Vesicle Fission(McMahon)Ion Channels and Transient Ion Channels and Transient PermeabilityPermeabilityChannels open in response to a variety of different stimuli.Key mechanisms are voltage gating, ligand binding-induced gating and mechanical tension in the membrane.Physics of Viral BuddingPhysics of Viral
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