1 MCDB 141 Molecular Basis of Plant Development and Differentiation Spring, 2005 Lectures: Monday and Wednesday, 11:00 - 12:15, Botany 325 Prerequisites: LS3 and LS4 or equivalent. Instructor: Professor Elaine Tobin Life Sciences 3313 Office Hours: Wednesdays, 3:00-4:00 or by appointment Email: [email protected] Tel: 825-7700 Teaching Assistant: Maskit Maymon Life Sciences 3326 Office Hours: Tuesday 2:00-3:00 P.M. or by appointment Email: [email protected] Tel: 825-6298 Discussion Sections: Geology 6704 Fridays: 9:00-9:50, 12:00-12:50, 2:00-2:50 Attendance at and participation in the discussion section is required and is an integral component of the course. During the discussion sections, students will discuss and critically analyze the assigned scientific research papers which will be available on the website. You need to come prepared. There will be a short assignment about the assigned research paper to be turned in at the beginning of each section. There is no textbook for this course. Reading material and copies of the research papers for discussion in section will be made available on the course web site: http://www.lsic.ucla.edu/classes/spring05/ Grading: Term Paper: 10% A term paper critically analyzing a research article is due in section on June 10. Further information about this assignment will be distributed in section. Discussion Section: 15% Midterm: 30% Final exam: June 16, 8 A.M. (Cumulative): 45% Please note: The exams will include material covered only in the discussion sections as well as lecture material.2 MCDB 141 Molecular Basis of Plant Development and Differentiation Spring, 2005 Date Lecture Topic April 4 1 Introduction and overview of plant development; plant cells and differentiation; plant life cycles April 6 2 Review of gene regulation and methods to study it for understanding plant development April 8 Section 1: Organization; introduction to reading research papers April 11 3 Plant transformation: the Ti plasmid of Agrobacterium tumifaciens; introduction to plant hormones and their roles in development April 13 4 Auxin and growth; phototropism and geotropism April 15 Section 2: Using computers: searching for articles; analyzing sequence data Meet in Young Hall Science Learning Center Room 4336 for 9 A.M. and 12 noon sections and in Room 4330 for the 2 P.M. section April 18 5 How plant cells enlarge—cell walls and auxin action April 20 6 Roles of auxin in development April 22 Section 3: Yang et al. (2004) The IAA1 protein is encoded by AXR5 and is a substrate of SCFTIR1. Plant J. 40: 772-782. April 25 7 Seed development and storage proteins, a role for abscisic acid April 27 8 Seed germination and early seedling growth, role of gibberellins April 29 Section 4: Yamauchi et al. (2004) Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. Plant Cell 16: 367-378. May 2 9 Seedling growth: introduction to the roles of light; the development of chloroplasts and photosynthesis May 4 10 Guest Lecturer: Dr. Chentao Lin, U.C.L.A. Roles of cryptochromes in plant growth and development May 6 Section 5: Shalitin et al. (2002) Regulation of Arabidopsis cryptochrome 2 by blue-light-dependent phosphorylation. Nature 417: 763-767.3 May 9 MIDTERM4 Date Lecture Topic May 11 11 The phytochrome family of photoreceptors: discovery, spectral properties, physiological effects May 13 Section 6: Franklin et al. (2003) Phytochromes B, D, and E act redundantly to control multiple physiological responses in Arabidopsis. Plant Physiol. 131: 1340-1346. May 16 12 Phytochrome action continued May 18 13 Guest Lecturer: Dr. Winslow Briggs, Carnegie Institution, Stanford Phototropins and their roles in plant development May 20 Section 7: Christie et al. (2002) Phototropin LOV domains exhibit distinct roles in regulating photoreceptor function. Plant J. 32: 205-219. PLUS deadline for submiting plan (a complete copy of article selected) for approval for your term paper (this can be done earlier if you would like). May 23 14 Guest Lecturer: Dr. Gary Gardner, University of Minnesota Floral development and hormonal regulation of flowering May 25 15 Environmental and genetic controls of flowering May 27 Section 8: Ayre and Turgeon (2004) Graft transmission of a floral stimulant derived from CONSTANS. Plant Physiol. 135: 2271-2278 May 30 MEMORIAL DAY HOLIDAY June 1 16 Guest Lecturer: Professor June Nasrallah, Cornell University Self incompatibility June 3 Section 9: Nasrallah et al. (2004) Natural variation in expression of self- incompatibility in Arabidopsis thaliana: Implications for the evolution of selfing. Proc. Natl. Acad. Sci. USA 101: 16070-16074. June 6 17 Circadian rhythms in plants and their importance for flowering June 8 16 Guest Lecturer: Professor Gary Gardner Plants and human health5 June 10 Section 10: Review for final and TURN IN TERM PAPER Final Examination June 16, 8