Lecture 6 Microbial cultivation and isolation BIO119 I. Isolating a pure culture A. Sample consideration 1. Environmental samples 2. Clinical samples 3. Microbial communities 4. Mixed populations B. What kind of tools to we need? 1. Incubators (shaking/non-shaking) 2. Petri dishes 3. Inoculating loop 4. Bunsen burner 5. Test tubes 6. Sterilization equipment 7. Sterile pipettes 8. Chemicals and water C. Streak plate procedures (Fig. 5.10)Lecture 6 Microbial cultivation and isolation BIO119 D. Spread plate procedures (Fig. 5.11A and 5.11B) E. Pour plate (Fig. 5.11C) F. Anaerobic methods (Fig. 5.12) G. Maintaining stock cultures (Fig. 5.13)Lecture 6 Microbial cultivation and isolation BIO119 II. Ways to isolate a pure culture by enrichment A. First figure out what type of organism you want to isolate. B. Design the growth medium. C. Define the strategy of isolation (see A above) D. Selective culturing on glucose (Fig. 5.14) III. Example #1: The hunt for a haloalkaliphilic archeaon that respires arsenate. A. What is arsenate respiration? B. Why look for this organism (hint: exobiology)? C. What did our team do? D. Were we successful? IV. Example #2: Isolation of a facultative anaerobic arsenate respiring bacterium A. Why did we look for this type of organism? B. Where did we look? C. Our success
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