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Biology 220 Summer II 2007 Lab syllabus This protocol contains a list of the experiments that will be performed each lab period. The experimentsare designed to provide students with the best possible opportunity to have a successful learningexperience. The protocols may not follow the procedures outlined in the lab manual some substitutionsfor organisms may be made. That information will be provided by the lab instructor. Students will beresponsible for understanding the principles set forth in each experiment. In addition text book readingsfrom Microbiology A Human Perspective by Nester 4th edition are listed which will further explain andhelp you understand the principles in these experiments.Laboratory Instructor: __________________________________ Office/Laboratory: __________________________________ Office phone: __________________________________ Office hours: __________________________________E-mail address: __________________________________June 25Lab 1 Lab safety protocolAseptic TechniquePure culture techniqueBacterial DistributionDiscuss: Genus and species; and morphologyTextbook readings: Pg 83-4 Section 4.1 Obtaining a Pure Culture read through The Streak-PlateMethod Pg 111-2 Microbiology LaboratoriesPg 250-1 Culture CharacteristicsPg 11 NomenclaturePg. 246-7 Taxonomic HierarchiesPg A-4 Appendix III Pronunciation KeyPg G1-18 GlossaryJune 26Lab 2 Complete aseptic technique; pure culture technique; bacterial distributionBright-field MicroscopyMaking a SmearSimple stainGram stainTextbook readings: Pg 40-1 Section 3.1 Microscopic Techniques: The Instruments read throughContrastPg 282 The Genus Corynebacterium Pg 45-7 Microscopic Techniques: Dyes and Staining read up to Acid-Fast StainPg 49-51 Section 3.3 Morphology of Prokaryotic CellsPg 248-9 Microscopic Morphology read through special stainsPg 58-9 The Gram Positive Cell Wall and the Gram Negative Cell Wall read up to the lipopolysaccharide moleculePg 61-2 Differences in Cell Wall Composition and the Gram StainJune 28Lab 3 Complete simple stain and gram stainCapsule stainSpore stain: Schaeffer-Fulton methodAcid-fast stain: Ziehl-Neelsen method******* streak plate and gram stain practiceTextbook readings: Pg 249 Special StainsPg 63 Section 3.7 Surface Layers External to the Cell WallPg 47 Capsule StainPg 470 Avoiding Recognition and Attachment (Capsules)Pg 48 Endospore StainPg 67-9 Endospores read up through Microcheck 3.9Pg 283 Endospore-FormersPg 698-703 Lockjaw and Gas GangrenePg 47 Acid-Fast StainPg 670-2 Hansen’s Disease (Leprosy)July 2Lab 4 Quiz 1 (Labs 1-3)Complete capsule stain, spore stain, and acid-fast stainEffect of Temperature, pH, and Salt concentration on bacterial growthEffect of Ultraviolet light on cell viability******* Streak plate for a gradeTextbook readings: Pg 86 Section 4.3 Environmental Factors that Influence Microbial GrowthPg 23-4 pHPg 99 Measuring Biomass (Turbidity)Pg 54 Permeability of the Cytoplasmic MembranePg 293-4 Section 11.9 Archaea that Thrive in Extreme ConditionsPg 122-3 Section 5.6 Using Radiation to Destroy Microorganisms and VirusesPg 196 Section 8.4 Repair of Damaged DNAJuly 3Lab 5 Hand in Laboratory Reports (Labs 1-3)Complete temperature, pH, and salt concentrationComplete ultraviolet lightAtmospheric Growth Requirement testingBacterial population counts (Spread and Pour Plates Methods) ******* Gram stain for a gradeTextbook readings: Pg 98-9 Plate CountsPg A-1 Appendix I Microbial MathematicsPg 88-9 Oxygen (O2) RequirementsPg 94-5 Providing Appropriate Atmospheric ConditionsPg 268-276 Section 11.1 Anaerobic ChemotrophsPg 281-2 Section 11.5 Aerobic ChemoorganotrophsJuly 5Lab 6 Complete spread and pour plates; atmospheric growthDifferential and selective mediaStaphylococciStreptococciTextbook readings: Pg 93-4 Section 4.5 Cultivating Prokaryotes in the Laboratory read up Providing Appropriate Atmospheric ConditionsPg 290 Section 11.8 Bacteria that Inhabit the SkinPg 372-6 Section 15.1 Overview of the Innate Defenses and Section15.2 First- Line DefensesChapter 22 Skin Infections and Chapter 23 Respiratory SystemPg 641-3 Staphylococcal Toxic ShockPg 693-6 Section 27.2 Common Bacterial Wound InfectionsJuly 9Lab 7 Quiz 2 (Labs 4-5)Complete Staphylococci; StreptococciContinue differential and selective mediaDental CariesPreparation and care of stock culturesGram Positive Cocci UnknownsCatalase productionCoagulase productionNovobiocin sensitivityTextbook readings:Pg 601-4 Section 24.3 Bacterial Diseases of the Upper Alimentary SystemPg. 84 Maintaining Stock CulturesJuly 10Lab 8 Continue identification of Gram Positive Cocci UnknownBacitracin, CAMP, SXT, Enterococcosel, 6.5% NaCl, and Optochin**NOTE** Any students that wants to bring in a water sample to test please collect sampling bottle from instructor. July 12Lab 9 Quiz 3 (Labs 6-8)Hand in Laboratory Reports (Labs 4-6)Continue identification of Gram Positive Cocci UnknownDetecting Coliform Bacteria in water: Multiple tube methodBacterial Analysis of foodMotilityTextbook readings: Pg 791 Section 31.2 Drinking Water Treatment and TestingPg 99 Most Probable Number (MPN)Pg 112 Foods and Food Production FacilitiesPg 114-5 Read Pasteurization through The Commercial Canning ProcessPg 123-4 Section 5.7 Preservation of Perishable ProductsPg 474 SuperantigensPg 610-621 Section 24.5 Bacterial Diseases of the Lower Alimentary System up toHepatitis APg 672-4 BotulismPg Chapter 32 Food MicrobiologyPg 63-6 Section 3.8 Filamentous Protein AppendagesPg 74 Flagella and CiliaJuly 16Lab 10 Complete motility experimentComplete identification of Gram Positive Cocci UnknownContinue water experimentComplete food experimentBiochemical testsIdentification of Gram negative unknown**NOTE**Hand out vials for students to bring in mouth wash for next periodTextbook readings: Pg. 251 Biochemical TestsPg 29-31 Section 2.5 Carbohydrates Pg 25 Section 2.4 Proteins and Their FunctionsThe media in these experiments have been pre-inoculated to provide both a positive and negative testresult. Students are to observe and record the reactions and where appropriate will perform certain testswith the reagents provided. Tests will be available for observation over the next lab period. 1 set-up/3students. Also: See Biochemical charts 1-8 in laboratory manual and the color pictures in the illustrationbinders.Carbohydrate fermentation:phenol red lactose blankE. coli in phenol red lactoseP. vulgaris in phenol


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IPFW BIOL BIOL220 - lab syllabus summer

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