MIT HST 410J - Manufacturing a PDMS microfluidic device via a Silicon Wafer Master (21 pages)

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Manufacturing a PDMS microfluidic device via a Silicon Wafer Master



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Manufacturing a PDMS microfluidic device via a Silicon Wafer Master

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Pages:
21
School:
Massachusetts Institute of Technology
Course:
Hst 410j - Projects in Microscale Engineering for the Life Sciences
Projects in Microscale Engineering for the Life Sciences Documents

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Harvard MIT Division of Health Sciences and Technology HST 410J Projects in Microscale Engineering for the Life Sciences Spring 2007 Course Directors Prof Dennis Freeman Prof Martha Gray and Prof Alexander Aranyosi Manufacturing a PDMS microfluidic device via a Silicon Wafer Master Eric Lam Tri Ngo Abstract This document describes the manufacturing process for a general microfluidic device which uses either pipette tips O Ring or non O Ring syringe reservoirs as a fluid delivery mechanism The manufacturing process does not involve much labor but requires a lot of waiting The entire process takes a minimum of 5 hours but optimally the process takes 9 hours First the PDMS is mixed and degassed for approximate 1 hour Then the activated PDMS is poured onto the master and baked in an oven After a minimum of 4 hours of baking the PDMS is unmolded from the master and processed further Next a glass slide is cleaned and the PDMS layer containing the microfluidic features are bonded to the glass slide Finally the device is completed by attaching either pipette tips or pre cut syringe tubes Mixing the PDMS In preparation for this stage obtain a some unpowdered disposable gloves a disposable plastic knife and a disposable plastic cup PDMS should be mixed in a 1 10 ratio of curing agent and PDMS monomers The PDMS monomers are much more viscous than the curing agents For this example implementation we will be using a mixture which consists of 7g of curing agent and 70g of monomer The PDMS can be mixed in a disposable plastic cup not unlike the cups found near water coolers Wider cups may be better because they expose a larger surface of the contained fluid to air which will aid in degassing the PDMS later While PDMS is very safe it is a sticky and messy substance to work with You should put on some disposable gloves before touching the containers or tools which come into contact with uncured PDMS Use unpowered gloves to prevent contaminating the PDMS At this stage you should not



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