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Hormone Induced Endoreduplication Prior to Mitosis in Cultured Pea Root Cortex Cells Author s Kornelis R Libbenga and John G Torrey Source American Journal of Botany Vol 60 No 4 Apr 1973 pp 293 299 Published by Botanical Society of America Stable URL http www jstor org stable 2441195 Accessed 23 08 2011 15 41 Your use of the JSTOR archive indicates your acceptance of the Terms Conditions of Use available at http www jstor org page info about policies terms jsp JSTOR is a not for profit service that helps scholars researchers and students discover use and build upon a wide range of content in a trusted digital archive We use information technology and tools to increase productivity and facilitate new forms of scholarship For more information about JSTOR please contact support jstor org Botanical Society of America is collaborating with JSTOR to digitize preserve and extend access to American Journal of Botany http www jstor org Amer J Bot 60 4 293 299 1973 HORMONE INDUCED ENDOREDUPLICATION PRIOR TO MITOSIS IN CULTURED PEA ROOT CORTEX CELLS KORNELIS R LIBBENGA2 AND JOHN G TORREY Cabot Foundation Harvard University Petersham Massachusetts01366 A B S T R A C T corticalexplantsexcisedasepticallyfrom10 11 mm behindthe tip of 3 dayOne mm thick old rootsof the gardenpea Pisum sativumn cv LittleMarvel were culturedon a synthetic with auxin or auxin and cytokinin Nuclear DNA contents nutrientmediumsupplemented weremeasuredin cells of theexplantsat theoutsetand at specifiedtimesduringcultureup to sevendays Fixed and sectionedpreparations werestainedwiththe Feulgenmethodusingthe measurements of indiDNA specificdye auramin O Fluorescentmicrospectro photometric vidual nucleiwere made fromeach corticalpopulation At day zero all corticalnuclei measuredwereeither2c or 4c withrespectto theirDNA content In the presenceof the auxins indoleaceticacid and 2 4 dichlorophenoxyacetic acid and the cytokinin kinetin DNA values increasedto multiplesof the 2c level withpopulationsat the 8c and



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