View Full Document

6 views

Unformatted text preview:

REAGENT ACQUSITION AND STORAGE You decide you want to try a particular experiment An idea you had An idea you got looking the literature An idea you had with your colleagues Etc You determine what reagents you will need to perform your experiment If you do not have all the reagents you must therefore obtain any missing reagents QUESTION 1 How much reagent do I need To answer this question you must first determine How much reagent is used per rxn How many rxns in total per experiment How many experiments do you intend to run If you are duplicating a rxn in the literature you use the amount given per rxn times the of desired rxns times the of experiments you plan to run You thus order at least a little more than this total For example if the literature called for 5 M ACTH1 39 in a 50 L final vol and you want to run 10 rxns per experiment and you wish to do 2 sets of experiments you would need 50 L rxn 5 mol 106 L 103 nmol mol 10 rxns experiment 2 experiments 5 nmol ACTH1 39 total The reagent concentration per rxn may be given in international units I U radioactivity weight or mass per unit volume weight or mass per unit mass etc In these cases you may need to convert to the relevant units to perform the necessary calculation s For example the MW of ACTH1 39 4567 Daltons 1 Da 1 g Thus 1 mol of ACTH1 39 weighs 4567 grams 1 mol 6 023 x 1023 molecules You may thus need to convert to the appropriate units 1 REAGENT ACQUSITION AND STORAGE Now you know how much reagent you will need initially QUESTION 2 What increments are available for purchase of the desired reagent To answer this question you must Look to the manufacture Compare different manufactures Pick one QUESTION 3 How much reagent to order To answer this question you must consider Reagent cost Reagent stability You find that no one sells the small amount necessary for your preliminary experiments Thus you decide to buy the least amount available for purchase among the relevant suppliers Manufacture reliability



Access the best Study Guides, Lecture Notes and Practice Exams

Loading Unlocking...
Login

Join to view REAGENT ACQUISITION AND STORAGE and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view REAGENT ACQUISITION AND STORAGE and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?