BIOCHEMISTRY AND MOLECULAR BIOLOGY OF THE PHOTOSYNTHETIC APPARATUSChem and Biochem and MCDB 170, 2 units onlyLecture: Tuesdays and Thursdays 9 - 9.50 in 2200 Young Hall, see schedule for specific datesDiscussion: Wednesday 8 - 8.50 in 2200 Young Hall, see schedule for specific datesOffice hours: walk in anytime, email ([email protected]) or telephone 5-8300Recommended text: Biochemistry and Molecular Biology of Plants, Buchanan, Gruissem, Jones. This course will introduce you to the primary scientific literature dealing with photosynthesis andchloroplast function, a topical area of research in plant biology. The biochemistry of thephotosynthetic apparatus will be the focus of our discussions. The lectures will give you thebackground you need to understand and appreciate research problems in this area. The purpose of thediscussions will be to get you involved in reading and understanding the research literature. The grade in this course will be based on 5 in-class quizzes or homework assignments (100 points)plus a final examination (100 points). The final examination will consist of questions that test yourunderstanding and interpretation of lecture material plus an evaluation of a paper from the researchliterature, which will draw on the experience you have gained via discussion of the primary literaturein class.I recommend that you read the text book before class and use the review articles to supplement the textbook material as needed. The review articles will help you to gain a broad perspective of the topicsyou will cover in the discussion of journal articles. The review articles will also help you to fill inbackground material that you might need in order to understand the research article. Read the parts ofthe review articles you need in order to understand the research article. Especially relevant ones aremarked with an asterisk. The journal articles can be found by following the links on the voh site forthis class.April 3 – Introduction by Dr. Beth Dreyfuss Buchanan textSection 1.9 and 1.10April 4 – no meetingApril 5 – 4345 Young Hall, Science learning center, lecture by Marion Peters on use of electroniclibrary resources for the primary scientific literatureApril 10-12 – Introduction to the plant cell, chloroplast and chloroplast genome, gene expressionBuchanan textSection 6.5.1 to Section 6.5.4,Section 6.6.2, Sections 6.8.1, 6.8.6, 6.8.7, Section 9.3Reviewshttp://www.plantphysiol.org/cgi/content/full/118/3/715 - envelopeshttp://www.plantphysiol.org/cgi/reprint/118/3/715.pdf*plastid proteomics – pdf on voh*Clp proteases 1 Clp proteases 2 – pdf on vohPapers for discussionhttp://www.jbc.org/cgi/reprint/M010503200v1.pdf - Clp proteasesApril 17–19 Electron transfer pathway, PSII, cyt b6/f and PSI Buchanan text Sections 12.1 to 12.7Papers for discussionhttp://www.emboj.org/cgi/reprint/19/10/2139.pdf - Crd1http://www.emboj.org/cgi/content/full/19/10/2139?http://www.jbc.org/cgi/reprint/274/43/30540.pdf - quinone binding proteinhttp://www.jbc.org/cgi/content/full/274/43/30540?April 24 - 25 – photosynthetic carbon reduction, Rubisco, photorespirationBuchanan textSection 12.8, Section 14.7 to 14.9Reviewhttp://plant.annualreviews.org/cgi/content/full/50/1/27http://plant.annualreviews.org/cgi/reprint/50/1/27.pdf - on phosphate translocatorApril 26 - May 1 – C4 assimilation and CO2 concentrationBuchanan textSection 12.9Reviewhttp://plant.annualreviews.org/cgi/content/full/50/1/539 - CO2 concentrationhttp://plant.annualreviews.org/cgi/reprint/50/1/539.pdf Paper for discussionhttp://www.emboj.org/cgi/reprint/16/22/6713.pdf - ycf10http://www.emboj.org/cgi/content/full/16/22/6713?May 2 -3 – Fate of triose phosphate — starch and sucrose biosynthesisBuchanan textSection 13.2Reviewshttp://www.plantphysiol.org/cgi/reprint/122/4/989.pdfhttp://plant.annualreviews.org/cgi/content/full/48/1/67http://plant.annualreviews.org/cgi/reprint/48/1/67.pdf starch pdf on vohResearch articlehttp://www.jbc.org/cgi/reprint/271/27/16281.pdf- ADPglucosehttp://www.jbc.org/cgi/content/full/271/27/16281?May 8 - 10 Chlorophyll biosynthesisBuchanan textSection 12.2.3Research articlehttp://www.plantcell.org/cgi/reprint/12/4/559.pdf - yellow in the dark mutanthttp://www.plantcell.org/cgi/content/full/12/4/559?May 15 - 17 Carotenoid biosynthesisBuchanan textSection 24.1 to 24.3, Section 12.2.4Reviewdeoxyxylulose pathwayhttp://plant.annualreviews.org/cgi/content/full/49/1/557http://plant.annualreviews.org/cgi/reprint/49/1/557.pdf Research articlehttp://www.sciencemag.org/cgi/reprint/282/5396/2098.pdf - Vitamin Ehttp://www.sciencemag.org/cgi/content/full/282/5396/2098?Discussion topichttp://www.sciencemag.org/cgi/reprint/285/5426/375.pdf - nutrition / healthhttp://www.sciencemag.org/cgi/content/full/285/5426/375?http://www.plantphysiol.org/cgi/reprint/125/3/1157.pdf - golden ricehttp://www.plantphysiol.org/cgi/content/full/125/3/1157metabolic engineering pdf on vohExam: to be