Biology 3A Laboratory Names:A. USING THE MICROSCOPEDetermining the Magnifying Power:Total Magnification = Ocular magnification X Objective magnificationMagnification Ocular Lens X Objective Lens = Total 1a. Scanning Power: X = 1b. Low Power: X = 1c. High Power (Dry): X = 1d. Oil Immersion: X = Image Orientation and Focusing:2. Describe the orientation of the letter “e” when viewed under the microscope.3. What direction in the field of view did the letter “e” move when you moved theslide slightly to the left or right using the slide movement knobs? Up or down?4. Describe the differences when viewing the letter “e” under the three differentmagnifications.5. What happened to the light intensity when you switched from low to high power?6. What can you do to adjust for this change?7. What happened to the working distance (distance between the slide and theobjective lens) when you increased magnification from 40X  100X  430X?Diameter of the Field of View:8. Scanning power diameter: 9. Low power diameter: Bio 3A Lab. Scopes & Cells Page 1 of 410. Calculate the high power diameter: Show your work.11. Convert the high power diameter to μM. Show your work.12. What are the advantages of knowing the diameter of the field of view at a givenmagnification?Calibration of the reticle:13. Using the 4 x objective, what is the length of 2mm in reticle units?a. Divide 2 mm by your measurementb. What is the length of one reticle unit at 40x?14. Using the 10 x objective, what is the length of 1mm in reticle units?a. Divide 1 mm by your measurementb. What is the length of one reticle unit at 100x?15. What is the length of one reticle unit at 400x?a. In mm?b. In μM?Depth of Field:16. Are all three colored threads in focus at low power?17.Why should you always focus an object on a lower power before focusing onhigh power?B. PROKARYOTIC AND EUKARYOTIC CELLS18.Clearly draw and label the different morphological bacterial types.Bio 3A Lab. Scopes & Cells Page 2 of 419.Draw the structure of Nostoc and Anabaena20.Clearly draw and label all visible components (cell membrane, cytoplasm,nucleus, nuclear envelope, mitochondria and nucleolus) of your cheek cell.What is the average size of your cheek cells?What is the approximate size of the mitochondria?21.Clearly draw and label all visible components (cell wall, cytoplasm, nucleus,nuclear envelope, mitochondria and nucleolus) of an onion cell.22.Clearly draw and label all visible components (cell wall, cytoplasm, chloroplastand area of vacuole) of an Elodea cell.22a. Can you see nuclei in the Elodea cells? Which are larger, chloroplasts ornuclei?22b. What is the speed of a chloroplast?23.Clearly draw and label all visible components of your protists. Include any otherprotests that are provided by you instructor.Bio 3A Lab. Scopes & Cells Page 3 of 424.If your lab partner missed today’s lab, how would you tell them to distinguishplant cells from animal cells when viewed through a microscope.21. Briefly explain why the onion leaf did not possess any chloroplasts.C. UNKNOWN IDENTIFICATION22. Unknown No.: 23. Write a short report on the overall description of the cell type you observed. Based on the above, my unknown specimen is a: ProkaryoteEukaryote(Circle One)If the specimen is a eukaryote, it is a(n): Plant Animal ProtistBio 3A Lab. Scopes & Cells Page 4 of