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Vitamin C Report Form Name____________________________ Part A What is concentration of the standard vitamin C solution? What is the alternative name for vitamin C? How did you investigate the effect of pH on the redox reaction between vitamin C and 2,6-dichlorophenol(2,6-DCIP)? Using your data, describe the effect of pH on the redox reaction between vitamin C and 2,6-DCIP. Complete the following table: Solutions Containing Vitamin C ~pH Observations when titrated with 2,6-DCIP(color of endpt.?) #drops to reach endpt. Changes to Solutions over time? Vit. C Vit. C + NaOH Vit. C + H2SO4 Vit. C + Fe2++ H2SO4Consider the collected data in the previous table and answer the following questions. Make a comparison of the first three titrations. Draw a conclusion from your results. Why is iron(II) ion being adding to the titration scheme? Compare the two titrations that included sulfuric acid and draw a conclusion. Write a balanced redox reaction for the last titration (Vit. C + Fe2++ H2SO4 reacting with 2,6-DCIP). What types of commercially-available products are likely to contain both vitamin C and iron(II) ion? Part B. How does the titration technique in Part A differ from the titrations in Part B? How many drops of 2,6-DCIP were required to react completely with 2 drops of vitamin C and 1 drop of sulfuric acid? Calculate how many drops of 2,6-DCIP will oxidize the vitamin C in 1 drop of the standard vitamin C solution. Show your work. Consider the concentration of the standard vitamin C solution. Consider the two methods of determining the volume delivered by your large drop microburet. Is one method preferred over the other? Why or why not?Which method did you use to calibrate your large drop microburet? What is the volume of one drop from your large drop microburet? Calculate the number of mg of vitamin C per drop. Calculate the number of mg of vitamin C oxidized by 1 drop of 2,6-DCIP. Part C. Is the titration technique in Part C the same as Part A or Part B? How many drops of 2,6-DCIP were required to reach an endpoint with the vitamin C solution of unknown concentration? What is the concentration of the unknown? Calculate the number of mg of vitamin C in the table used to make the solution. Part D. How did you prepare your juice samples? Complete the following table: Type of Juice #drops 2,6-DCIPto reach endpoint Color of endpoint Vitamin C conc. of fruit juice (mg/mL) Total vitamin C content of fruit (mg/mL) What happened when you titrated the unfiltered juice?Part E. Describe the procedure you used to analyze the breakfast cereal for vitamin C content. Include the type of cereal analyzed. How many mg of vitamin C are in a 1 oz. serving of this cereal? Compare your results to the information given on the box and the RDAs provided in the introductory section of this experiment. Draw conclusions regarding the reliability of your results and the nutritional content of the


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CABRILLO CHEM 1B - Vitamin C Report Form

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