ABIO 350 Fundamental Genetics Dr. Wm. JacksonSyllabus Spring 2001Instructor InformationInstructor:William H. Jackson, Ph.D.Office:SBDG 111BOffice Hours:By AppointmentTelephone:641-3601Email:[email protected] InformationLecture:TTH: 12:15-1:30Text:Concepts of Genetics, 6th Edition by Klug and CummingsLaboratory:TH: 3:05-5:45Text:Laboratory Manual of Genetics, 12th edition by MertensHammersmithSupplies:Scantron Sheets, Laboratory notebook, calculator and disketteAdditionalInformation:http://www.usca.sc.edu/biogeo/faculty/Jackson/Jackson.htmlCourse Description: Fundamental Genetics is a four credit hour course for biology majors that covers the principlesof transmission, molecular genetics, and population genetics. Topics covered will include quantitative inheritance;recombination; the biochemical aspects of gene function and regulation; and developmental and population genetics.Course Objectives: This course will introduce the student to the underlying principles of genetics. It is divided intotransmission and molecular genetics. On completion of this course, the student will be expected to1. Demonstrate an understanding of transmission genetics2. Demonstrate an understanding of molecular genetics3. Demonstrate an ability to write and discuss applications of genetics concepts4. Solve genetics problems using mathematical techniques in conjunction with data collected in the geneticslaboratory5. Describe and utilize the laboratory techniques of DNA isolation, PCR, and gel electrophoresisAttendance Policy: Students are expected to adhere to the University attendance policy as stated in the StudentHandbook. In this regard, the instructor may impose a penalty for absences in excess of 25% of regularly scheduledclass meetings by assigning an "F" for the course. Absences, neither excused nor unexcused, absolve the studentfrom meeting class assignments. Because of their nature, laboratory exercises cannot be made up - students shouldmake every effort to attend all laboratory sessions.Disability Statement: If you have a physical, psychological, and/or learning disability which might affect yourperformance in this class, please contact the Office of Disability Services, 126A B&E, (803) 641-3609, as soon aspossible. The Disability Services Office will determine appropriate accommodations based on medicaldocumentation.Methods of Presentation and Evaluation: Information will be presented through lectures, class discussion, andlaboratory exercises using appropriate visual aids and laboratory equipment. Your laboratory grade will make up25% of your final course grade (see below). For additional information on laboratory requirements, see theLaboratory Syllabus supplement.Description Percentage CommentsMidterm Exam 30% Comprehensive exam covering the first half of the semesterFinal Exam 30% Comprehensive exam covering the second half of the semesterWeekly quizzes 15% Quizzes will be taken on Tuesday's of each week and will cover thematerial of the previous weekLaboratory 25% See Laboratory Syllabus supplement for grading informationABIO 350 Fundamental Genetics Dr. Wm. JacksonSyllabus Spring 2001You will be expected to endorse the USCA HONOR PLEDGE on every assignment: 'On my honor as a Universityof South Carolina Aiken student, I have neither given nor received any unauthorized aid on thisassignment/examination. To the best of my knowledge, I am not in violation of academic honesty'.TENTATIVE LECTURE SCHEDULEWEEK DATE TOPIC CHAPTER1 1.11.01 An Introduction to Genetics 12 1.16.011.18.01Mitosis and MeiosisThe Cell Cycle223 1.23.011.25.01Mendelian GeneticsMendelian Genetics334 1.30.012.1.01Extensions of Mendelian GeneticsModified Mendelian Relationships445 2.6.012.8.01Linkage and Crossing OverMapping666 2.13.012.15.01Genetic Recombination in BacteriaGenetic Recombination in Bacteria777 2.20.012.22.01Sex DeterminationChromosomal Mutations9108 2.27.013.1.01DNA Structure and AnalysisMidterm Examination11---9 3.13.013.15.01DNA Structure and AnalysisDNA Replication111210 3.20.013.22.01DNA Replication and RecombinationGenetic Code121311 3.27.013.29.01TranscriptionTranslation131412 4.3.014.5.01TranslationNo classes14---13 4.10.014.12.01Regulation of Gene Expression in ProkaryotesRegulation of Gene Expression in Eukaryotes151614 4.17.014.19.01Regulation of Gene Expression in EukaryotesRegulation of Gene Expression in Eukaryotes161615 4.24.014.26.01Population GeneticsPopulation Genetics2525Final5.8.01 Final Exam at 11:00 AM in SBDG 200ABIO 350 Fundamental Genetics Dr. Wm. JacksonSyllabus Spring 2001InstructorWilliam H. Jackson, PhD.OfficeSBDG 111BOffice HoursBy [email protected]://www.usca.sc.edu/biogeo/faculty/jackson/jackson.htmlTextConcepts of Genetics, 6th Edition by Klug and CummingsLaboratoryManualLaboratory Manual of Genetics, 4th Edition by Winchester andWejksnoraAdditionMaterialsGrid-lined laboratory notebook, calculator, disketteRequiredReadingDrosophila Life Cycle | Fly Care | Anesthetizing Flies | Lab ReportGuidelinesLaboratory ScheduleMonth Date Laboratory Exercise ChapterJanuary 11 Introduction to Genetics Laboratory and Mitosis 1, 218 Probability 325 Random Segregation 4February 1 Independent Assortment 58 Sex-Influenced Inheritance 615 Linkage and Crossing Over 822 DNA FingerprintingMarch 1 DNA Fingerprinting Continued15 RFLP Analysis22 RFLP Analysis Continued29 Plasmid Mapping 13April 12 Plasmid Transformation 1419 Plasmid DNA Extraction from Transformed Cells 1526 Plasmid DNA Extraction Continued*Genes in Populations 20*Must be begun during Laboratory 3ABIO 350 Fundamental Genetics Dr. Wm. JacksonSyllabus Spring 2001General Information: The laboratory component of ABIO 350 is not separate from the lecturesessions. You are encouraged to synthesize the material covered in lecture, laboratory, and thetext. You are expected to meet every laboratory session, as it will be impossible to recreate thelaboratory setup after the normal meeting time. The laboratory has been created to introduce thestudent to genetics using living organisms (Drosophila melanogaster, Escherichia coli ).Because you are experimenting with living organisms, you must be prepared to work on theproject when the need arises. While the majority of the work can be done during normal meetingtimes, this is not always the case.Recording Data: One of the most critical requirements for any scientist is careful recordkeeping. In order to intelligently analyze and report your data, you should