BIOFILMS IN WATER BIOFILMS IN WATER DISTRIBUTIONDISTRIBUTIONBYBYYASHODHARA KAMBAMYASHODHARA KAMBAMCONTENTSCONTENTSIntroductionIntroductionDescriptionDescriptionWhat is a Biofilm?What is a Biofilm?What Kinds of Microorganisms Make Up the Biofilm?What Kinds of Microorganisms Make Up the Biofilm?Factors that Favor Biofilm GrowthFactors that Favor Biofilm GrowthDiscussionDiscussionHow to Recognize a Biofilm OccurrenceHow to Recognize a Biofilm OccurrenceBiofilm Control StrategiesBiofilm Control StrategiesConclusionConclusionIntroductionIntroductionThe occurrence of bacterial growths in finished drinking The occurrence of bacterial growths in finished drinking water is not new.water is not new.In 1930, the AWWA committee on water supply reported In 1930, the AWWA committee on water supply reported the problem of regrowth of the problem of regrowth of ““B. ColiB. Coli””..Bacteria could not be detected in the finished water at Bacteria could not be detected in the finished water at the point of entry; however, microorganisms were the point of entry; however, microorganisms were apparently multiplying in distribution pipelines.apparently multiplying in distribution pipelines.While water treatment and disinfection systems can While water treatment and disinfection systems can remove most of the bacteria found in raw water, the remove most of the bacteria found in raw water, the water produced is not sterile, and low levels of bacteria water produced is not sterile, and low levels of bacteria do persist even in properly treated supplies.do persist even in properly treated supplies.Bacterial growth in drinking water Bacterial growth in drinking water distribution systems makes monitoring for distribution systems makes monitoring for bacterial quality in the distribution system bacterial quality in the distribution system difficult.difficult.Growths of bacteria on pipe walls, called Growths of bacteria on pipe walls, called biofilms, also can provide a haven for biofilms, also can provide a haven for potentially pathogenic bacteria.potentially pathogenic bacteria.Biofilm is an organic or inorganic surface Biofilm is an organic or inorganic surface deposit consisting of microorganisms, deposit consisting of microorganisms, microbial products, and detritus.microbial products, and detritus.DescriptionDescriptionWhat is a Biofilm?What is a Biofilm?Biofilms are formed in distribution system Biofilms are formed in distribution system pipelines when microbial cells attach to pipe pipelines when microbial cells attach to pipe surfaces and multiply to form a film or slime surfaces and multiply to form a film or slime layer on the pipe. They are dynamic layer on the pipe. They are dynamic microenvironments, encompassing processes microenvironments, encompassing processes such as metabolism, growth, and product such as metabolism, growth, and product formation finally detachment, erosion, or formation finally detachment, erosion, or sloughing of the biofilm from the surface.sloughing of the biofilm from the surface.The biofilm may grow until the surface layers The biofilm may grow until the surface layers begin to slough off into the water, and the pieces begin to slough off into the water, and the pieces of biofilm released into the water may continue to of biofilm released into the water may continue to provide protection for the organisms until they provide protection for the organisms until they can colonize a new section of the distribution can colonize a new section of the distribution system.system.Macromolecules tend to accumulate at solidMacromolecules tend to accumulate at solid--liquid liquid interfaces, creating a favorable environment in interfaces, creating a favorable environment in otherother--wise nutrientwise nutrient--deficient situation.deficient situation.A high flow rate in the system can transport A high flow rate in the system can transport tremendous quantities of nutrients to fixed tremendous quantities of nutrients to fixed microorganisms, even when nutrient microorganisms, even when nutrient concentration in the water is low.concentration in the water is low.Production of EPS (extra cellular Production of EPS (extra cellular polysaccharides) helps to anchor attached polysaccharides) helps to anchor attached bacteria; EPS also may be a factor in nutrient bacteria; EPS also may be a factor in nutrient capture.capture.Bacteria embedded in EPS matrices are Bacteria embedded in EPS matrices are protected from disinfectants by a combination of protected from disinfectants by a combination of physical and transport phenomena.physical and transport phenomena.What Kinds of Microorganisms What Kinds of Microorganisms Make Up the Biofilm?Make Up the Biofilm?BacteriaBacteriaThe predominant coli The predominant coli form species form species generally include generally include Enterobacter cloacae, Enterobacter cloacae, Klebsiella, Citrobacter Klebsiella, Citrobacter freundii and freundii and Enterobacter Enterobacter agglomerans.agglomerans.FungiFungiFungi which include yeasts Fungi which include yeasts and molds can be found and molds can be found in finished water and can in finished water and can colonize and multiply in colonize and multiply in the pipe system. The the pipe system. The primary concerns for primary concerns for fungi in drinking water fungi in drinking water are taste and odor are taste and odor complaints.complaints.ProtozoaProtozoaBiofilms in potable Biofilms in potable water systems may water systems may contain a variety of contain a variety of nonpathogenic nonpathogenic protozoa and other protozoa and other invertebrates.invertebrates.Factors that favor biofilm growthFactors that favor biofilm growthHydraulic EffectsHydraulic EffectsNutrient AvailabilityNutrient AvailabilityCarbonCarbonNitrogen and PhosphorusNitrogen and PhosphorusOther Sources of NutrientsOther Sources of NutrientsDisinfection Residual ConcentrationsDisinfection Residual ConcentrationsCorrosionCorrosionSediment AccumulationSediment AccumulationDiscussionDiscussionHow to Recognize a Biofilm OccurrenceHow to Recognize a Biofilm Occurrence1.1.Detection of Breakthrough ContaminationDetection of Breakthrough Contamination2.2.Detection of BiofilmsDetection of BiofilmsCharacteristics of Biofilm ProblemsCharacteristics of Biofilm