Final Lab Practical Written Version Name100 pts. (All multiple choice worth 2 pts each).1. What is the total magnification of the microscope with the lenses in the current position?a. 4Xb. 40Xc. 10Xd. 100Xe. 1000X2. What is the name of the microscope structure marked by the arrow V?a. objectiveb. ocularc. condenserd. iris diaphragme. nosepiece3. Which objective in the list below focuses closest to the slide?a. 4Xb. 10Xc. 20Xd. 40Xe. 100X4. What is the function of the microscope structure above labeled VI.?a. move the objectives up and down in focusingb. focus the light from the lamp onto the specimenc. regulate the amount of light falling on the specimend. magnify the image from the specimene. increase or decrease the numerical apertureBio 41 Written Final Lab Exam pg 15. Examine the view of the slide under a microscope shown at the right. To what Kingdom does this organism belong?a. Fungib. Protistac. Animaliad. Monerae. Plantae6. Give the correct scientific term that describes the shape of these organisms in question 5:a. filamentousb. bacillusc. coccusd. ovoide. spirillum--------------------------------------------------------------------------------------------------------7. Which instrument or tool above is used to inoculate agar deeps? Answer A-E.8. Which tool or instrument above is commonly used to inoculate agar slants?--------------------------------------------------9. The same bacterial species was inoculated into several glucose fermentation tubes, shown at the right. What chemical substance probably caused the color change in tube B?a. acidb. acetoinc. ammoniad. oxidasee. catalaseBio 41 Written Final Lab Exam pg 210. In the glucose fermentation tubes above, Tubes A and B were inoculated at the same time, but have been incubating for different periods of time. How old would you predict the culture to be in Tube B?a. less than 1 hourb. 1 hourc. 10 hoursd. 24 hourse. 48 hours11. What are the characteristics of a bacterium that could cause the condition known as pharyngitis?a. catalase positive, oxidase negative coccib. beta hemolytic, bacitracin sensitive coccic. beta hemolytic, catalase positive coccid. gamma hemolytic, oxidase positive coccie. beta hemolytic, catalase negative bacilli12. What is the name of a bacterial species that causes pharyngitis?a. Staphylococcus aureusb. Streptococcus sanguisc. Proteus vulgarisd. Streptococcus mutanse. Streptococcus pyogenes13. A urine sample from a patient was diluted and plated on blood agar as shown in the diagram above. What is the concentration of bacteria in the patient’s urine?a. 2800 bacteria/mlb. 2.8 x 10-3 bacteria/mlc. 2.8 x 104 bacteria/mld. 2.8 x 105 bacteria/mle. 2.8 x 103 bacteria/ml14. What can you safely conclude about the patient above from whom the urine sample was taken?a. the person does not have a urinary tract infectionb. the person has E. coli in his/her urinec. the person has a Candida infectiond. the person has urinary tract infectionBio 41 Written Final Lab Exam pg 3e. the person has been eating too much sugar-------------------------------------------------------15. What is the purpose of flaming the mouth of a tube during bacterial transfers?a. to kill and decontaminate the culture inside the tubeb. to provide heat to enhance growth of transferred bacteriac. to cause upward air currents that prevent contamination when the tube is opend. to add heat so that the cap will seal properly on the tube tope. to warm the tube so that it is easier to hold16. Examine the streaking on the plate at the right. What is the purpose of streaking a plate in this fashion?a. to increase the concentration of bacteria on the plateb. to produce a “lawn” of bacterial coloniesc. to cover as much of the plate as possibled. to isolate single colonies by spreading the bacteria widelye. to cool off the loop gradually so the bacteria will survive17. A sucrose blood agar plate is inoculated with saliva. What type of bacterial product is being formed by the smooth, mucoid colonies?a. acetoinb. ethanol and CO2c. catalased. dextrane. amylase18.What two things are required for dental decay to occur?a. lactose and bacteriab. acid and basec. blood and living tissued. bacteria and sucrosee. glucose and bacteria19. What type of medium is shown at the right, when used with respiratory tract bacteria?a. selective (only)b. enriching (only)c. differential (only)d. selective and differentiale. differential and enriching20. What metabolic activity is seen on thisBio 41 Written Final Lab Exam pg 4plate above in question 19?a. alpha hemolysisb. mannitol fermentationc. starch hydrolysisd. gamma hemolysise. beta hemolysis21. The best kind of stains that sick to bacterial cells are usually:a. nigrosinesb. basicc. acidicd. neutrale. negative22. In the hanging drop procedure, what was the purpose of laying down a line of water or vaseline around the edge of the coverslip?a. to get the organisms to stick to the slideb. to create a depression for the droplet to hang inc. to prevent evaporation of the dropletd. to slow down the movement of the organisms in the droplete. to provide food to the specimen so that it lives longer23. The agar slant medium at the right was used to study and separate bacteria that cause urinary tract infections. This medium can indicate the production of which of the following bacterial enzyme(s)?a. catalaseb. sugar fermentation enzymes Urea agarc. sugar oxidation enzymesd. peptone degrading enzymese. urease24. What substance causes the color change in the inoculated tube shown in question 23?a. ammoniab. lactic acidc. pyruvated. acetoine. urea25. The growth medium at the right is designed to select for a bacterial species that lives in which part of the healthy human body?a. mouthBio 41 Written Final Lab Exam pg 5b. gastrointestinal tractc. upper respiratory tractd. skine. urogenital tract Tomato Juice Agar26. Which of the following is TRUE about the organisms that grow best on the medium shown in question 25?a. they are gram negativeb. they are spirilla shapedc. they produce coagulased. they are catalase negativee. they produce dextran or levan27. In the staining procedure using the substances below, which substance acts as the mordant? Answer A-E.28. A slide has just received a transfer of bacteria into a few drops of water, and has dried. What is thevery next step you would take, assuming you wished to stain the bacteria with a simple stain?a. add the stainb. heat fixc. wash with ethanold.