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UB BIO 201 - BIO 201 Exam 2

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Exam II, BIO201B, 2005 Form 21. Protein A has a net overall positive charge at pH=7.0. It is water soluble and 200kd. When this protein in placed on a polyacrylamide gel (with no SDS present) in an electric field:A. It would not move because there is no SDS present B. It would become insoluble, fall out of solution and not move C. It would move toward the negative pole (cathode)D. It would move towards the positive pole (anode)E. It would not move because it is too big2. The loss of mitotic spindles can be demonstrated by changing the temperature of fertilized sea urchin eggs because:A. Tubulin mRNA transcription is temperature dependent, causing less tubulin tobe made at low temperature B. The centriolar MTs of the centrosomes can depolymerize at low temperature (0 to 4oC) and repolymerize at 15oC.C. Cytoplasmic MTs are cold labileD. The low temperature activates certain proteases that destroy tubulins E. The dynamic instability of all MTs is seen more at 15oC than at low temperature (0 to 4oC)3. The head regions of cytoplasmic dyneins:A. All have an amino acid sequences that are quite similar, regardless of whether they come from protozoans or from human cellsB. Are tetramersC. Are also called S1D. Bind more to gamma tubulin than alpha tubulinE. Are required for microtuble sliding4. In the Weisenburg method for MT purification described in class:A. The P1 pellet should not contain any MTsB. The salt concentration must have been low because MAPS were found in theP2 pelletC. Axonemal MTs would be in the P2 fractionD. The only proteins in the S1 (first supernatant) fraction were tubulinsE. MFs would not be present in either of the pellets because they were depolymerized during the procedure5. Which of the following is false?A. A major assumption about SDS-PAGE is that many proteins are not water soluble or negatively charged unless SDS is addedB. Bacterial DNA does not contain any telomeric sequences C. Thick filaments, axonemes and proteins involved in intracellular transport all generate ADP when they are activeD. At 0 to 4oC, there are no protofilaments or microtubules in fertilized sea urchineggsE. During contraction of a sarcomere, the A-band doesn’t change much in lengthbut the distance between the Z-lines does change significantly16. Which of the following would be most likely to inhibit brain cytoplasmic microtubule formation from purified tubulin subunits in vitro?A. A calcium concentration that is too lowB. A GTP concentration that is too lowC. High temperature (98oF)D. Not enough light to permit increased light scatteringE. Anti-MAP antibodies7. One of the main questions asked in the Hershey-Chase experiments with metabolically labeled viruses was:A. Can DNA viruses replicate in bacterial hosts?B. Do some viruses contain RNA instead of DNA?C. Do viruses bring along their own enzymes for replication or do they use the enzymes of the host bacteria?D. Will infecting (parental) viruses pass their proteins on to their progeny?E. Can 32P be used to just label viral DNA without making their proteins radioactive?8. Fluorescent-conjugated proteins:A. Have alternating double and single bonds as part of their protein structure (between their amino acids)B. Can be used to fluorescently label completed centriolar MTs in a centrioleC. Are used in immunofluorescenceD. Cannot be seen by epi-fluorescence E. Are proteins with fluorescent compounds attached by covalent bonds9. Which of the following would stop both anterograde transport and the bending of fully formed axonemes?A. Colchicine B. Low ATP levelsC. High Ca++D. Low GTP concentrationsE. Anti-nexin antibodies 10. Intermediate filaments (IFs) are seen in eukaryotic cells:A. Only in the cytoplasm B. As the only cytoskeletal filaments left behind after Triton X-100 extraction of atypical eukaryotic cell like a fibroblastC. In both the nucleoplasm and cytoplasm D. As part of the axonemal structure necessary for cilia to beat properlyE. In the cytoplasm as “rails” for intracellular transport11. Axonemal microtubules (MTs) contain:A. Gamma tubulins at the plus ends B. Alpha and beta tubulin arranged as dimers in protofilaments and many protofilaments bound together to make A and B tubules C. Beta tubulins at the minus endsD. Bound ATP to provide the energy for ciliary movement E. 26 protofilaments 212. Which of the following are most likely to be the emission and excitation wavelengths for a fluorescent compound?A. Excite at 400nm, emit at 300nmB. Emission at 300nm when excited at 520nmC. Emission at 200nm when excited in the uv at 250nm D. Emission at 520nm when excited at 300nm E. Excite in the uv at 220nm and emit at 220nm13. The tail region of axonemal dynein binds to:A. Radial spokes B. TubulinC. Many different kinds of cargo in the axonemeD. NexinE. B tubules14. Which of the following was not said to be able to hydrolyze ATP in BIO201B?A. S1 fragmentsB. AxonemesC. Cytoplasmic dyneinD. KinesinE. Nuclear pore complexes15. Kinesins move cargo:A. As plus-end directed motors, moving cargo in the retrograde directionB. As minus-end directed motors, moving cargo in the anterograde directionC. As plus-end directed motors, moving cargo in the anterograde direction D. As minus-end directed motors, moving cargo in the retrograde directionE. As minus-end directed motors, moving cargo in both directions16. Kinetochores are:A. HeterochromatinB. Protein complexesC. Part of centrosomesD. “Rails” for chromosomal movementsE. Proteins associated with telomeres17. Complete ribosomes are functional in the _____________ and the ribosomal subunits are assembled in the ________________ from ________________ made in the _______________.A. Nucleus cytoplasm ribosomal proteins cytoplasmB. Cytoplasm nucleolus ribosomal proteins cytoplasmC. Cytoplasm nucleolus ribosomal proteins nucleolusD. Nucleolus cytoplasm rRNA nucleusE. Nucleus nucleolus rRNA cytoplasm18. Free, unbound importin will bind best to which of the following?A. Nuclear laminaB. Nuclear matrix C. Nuclear poreD. Nuclear envelopeE. Nuclear localization signal (NLS)319. Which of the following is true?A. The entire length of linear, eukaryotic DNA carries genetic information for making proteinsB. Telomers are only seen at the ends of eukaryotic DNA and RNAC. SER (smooth endoplasmic reticulum) is smooth because it is encapsulatedD. Microsomes can be separated into RER and SER fractions


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