BME 42-731 / ECE 18-795 Reading Assignment #2 Bioimage data analysis I: particle detection Assigned on Feb-24-2010, Due on March-15-2010 in class A. Overview The goal of this reading assignment is to review particle detection techniques for bioimage data analysis. B. Instructions Write a report that briefly reviews methods for particle detection and for achieving sub-pixel detection resolution. Many of these methods have been covered in lectures 8 & 9. Briefly comment on their strengths and limitations. Any or all of the following references may be used. (1) Reference articles [1] M. K. Cheezum, W. F. Walker, and W. H Guilford, Quantitative comparison of algorithms for tracking single fluororescent particles, Biophysical Journal, 81:2378-2388, 2001. [2] A. Ponti, P. Vallotton, W. C. Salmon, C. M. Waterman-Storer, and G. Danuser, Computational analysis of F-actin turnover in cortical actin meshworks using fluorescent speckle microscopy, Biophysical Journal, 84:3336-3352, 2003. [3] D. Thomann, D. R. Rines, P. K. Sorger, and G. Danuser, Automatic fluorescence tag detection in 3D with super-resolution, J. Microscopy, 208:49-64, 2002. Also, feel free to use any additional references you find useful. Be sure to cite properly. C. Report format The report, including references, must not be more than 3 pages. Reports longer than 3 pages can not be accepted. Page size: letter Line space: single Page margins: no less than 1 inch Font size: 12 points for the main text; 10 points for listed referencesD. General guidelines - Divide your report into sections/subsections and use section/subsection titles for clarity and readability. - Use your own words. Avoid literal replication of texts in references as this could run the risk of plagiarism http://www.studentaffairs.cmu.edu/acad_integ/acad_integ_text.html. - Be sure to use quotation marks to indicate literal