Biology 3A Laboratory Name: Mitosis Worksheet Lab Day & Time: A. PLANT AND ANIMAL MITOSIS1. Make representative drawings of the various distinct mitotic stages from both preparedslides of the onion root tip and whitefish blastula. Label (at least once) in both plant andanimal cells, if present: nucleus, chromosomes, chromatid, centrioles or asters, spindlefibers, cleavage furrow, cell plate, cytokinesis, karyokinesis.PLANT ANIMALInterphaseProphaseMetaphaseAnaphaseTelophaseBiology 3A Laboratory Mitosis- Asexual Reproduction (03/09) Page 1 of 32. List several major differences you have observed between plant and animal mitosis.B. MITOTIC STAGE FREQUENCY3. Data Table 1: Individual mitotic stage frequency for onion root tip.Stage # Observed - Tally Total # Observed FrequencyInterphaseProphaseMetaphaseAnaphaseTelophaseRECORD YOUR GROUP’S DATA ON THE COMPUTERHave your instructor sign you off – Instructor’s initials: 4. Data Table 2: Duration of mitotic stages.Phase Student Pair Lab Section All Sections# ofcells/stageDuration(min)# ofcells/stageDuration(min)# ofcells/stageDuration(min)Total # of nuclei# in interphase# in prophase# in metaphase# in anaphase# in telophase5. Estimate the duration of each stage based on your raw data, the mean lab section and themean data for all lab sections. The duration of each mitotic stage may be estimated usingthe following equation:Time/mitotic stage = number of cells/stage x 24 hr x 60 min total number of cells mitotic 1 hr6. Using Excel, graph the frequency of cells observed in each mitotic phase for your group’sdata, your cumulative lab section’s data and the entire cumulative data for all lab sectionson the same graph. 7. Based upon the data in Table 2 on the number of cells undergoing mitosis, what phase orstage of active mitosis is the longest for: - student pair - lab section - all sections Biology 3A Laboratory Mitosis- Asexual Reproduction (03/09) Page 2 of 38. Based upon the class data on the number of cells undergoing mitosis, what phase or stageof active mitosis is the shortest for:- student pair - lab section - all sections 9. The nuclear envelope/membrane disappears during what stage of mitosis? Explain why.C. Chromosome Staining Technique10. Were you able to determine the different mitotic stages in your slide? If you could not seeany chromosomes, what should you do?11. Data Table 3: Mitotic stage frequency for the fresh onion root tip. Instructor’s initials: Stage Total # Observed FrequencyInterphaseProphaseMetaphaseAnaphaseTelophase12. How does your data compare with section A?13. List several other types of stains that are used to stain chromosomes?Biology 3A Laboratory Mitosis- Asexual Reproduction (03/09) Page 3 of
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