Fingerprints: Nuclear DNA standard.PowerPoint PresentationSlide 3Slide 4Slide 5Fingerprints: Nuclear DNA standard.Fingerprints: Nuclear DNA standard.FBI CODIS (FBI CODIS (CoCombined mbined DDNA NA IIndex ndex SSystem) standard ystem) standard for nuclear DNA utilizes 13 highly-variable tetramer for nuclear DNA utilizes 13 highly-variable tetramer STR sites.STR sites.CSF1POD5S818D21S11TH01TPOXD13S317D7S820D16S539D18S51D8S1179D3S1358FGAVWAAMELAMELMultiplexed groupings:Multiplexed groupings: BlueBlue,, Green IIGreen II,, YellowYellow, & Cofiler, & CofilerD3 FGAvWABlueBlueD13D5D7YellowYellowA D8 D21 D18Green IIGreen IIAmpFlSTRAmpFlSTR®® Profiler Plus™ Profiler Plus™Select PCR primer sites at a distance around site to Select PCR primer sites at a distance around site to separate the products in each dye grouping:separate the products in each dye grouping:100 bp400 bp300 bp200 bpSize SeparationSize SeparationColor Color SeparationSeparationMultiplex STR AnalysisMultiplex STR AnalysisTwo different individualsTwo different individualsAAD19D19D3D3D8D8TH01TH01VWAVWAD21D21FGAFGAD16D16D18D18D2D2AAD19D19D3D3D8D8TH01TH01VWAVWAD21D21FGAFGAD16D16D18D18D2D2AmpFlSTR® SGM Plus™ kitMultiplex STR AnalysisMultiplex STR AnalysisMeasure against Reference LadderMeasure against Reference Ladder005510101515202025253030353540404545667788999.39.31010Caucasians (N=427)Caucasians (N=427)Blacks (N=414)Blacks (N=414)Hispanics (N=414)Hispanics (N=414)TH01 MarkerTH01 Marker**Proc. Int. Sym. Hum. ID Proc. Int. Sym. Hum. ID (Promega)(Promega) 1997, p. 34 1997, p. 34Number of repeatsNumber of repeatsFrequencyFrequencySTR Allele FrequenciesSTR Allele
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