DOC PREVIEW
Saddleback BIO 3A - Laboratory Photosynthesis

This preview shows page 1 out of 3 pages.

Save
View full document
View full document
Premium Document
Do you want full access? Go Premium and unlock all 3 pages.
Access to all documents
Download any document
Ad free experience
Premium Document
Do you want full access? Go Premium and unlock all 3 pages.
Access to all documents
Download any document
Ad free experience

Unformatted text preview:

Total O2 Production (mg/L)Species_______________________Pigments presentRf valuesSpecies_______________________Pigments presentRf valuesSpecies_______________________Pigments presentRf valuesB2. Spectral Analysis of Spinach ExtractLight SourceBiology 3A Laboratory Name:_________________PhotosynthesisA. Photosynthetic Photography1. Do you see the image in the leaf? Is the image a positive or negative of the original? Explain youranswer to this question.B1. Chromatography of Photosynthetic PigmentsDraw the results of your chromatogramsSpecies_______________________ PigmentspresentRf valuesSpecies_______________________ PigmentspresentRf valuesSpecies_______________________ PigmentspresentRf valuesFigure 3. Results of paper chromatography on three plant species2. How does the number of pigments vary among the three species?3. How does the type of pigment vary among the species? How can you account for this variation?B2. Spectral Analysis of Spinach ExtractComplete the following table Instructor initials Table One. Spectral Absorbance of a Spinach ExtractWavelength (nm) Absorbance Color 4004204404604805005205405605806006206406606807001. What colors of light are absorbed by the spinach pigments? 2. What colors do living spinach leaves reflect? C. Oxygen production during Photosynthesis. Table 2. Number and percentage of leaf discs floating after 10 and 20 minutes Instructor initials Light Source No. Floating at10 minutes% Floating at10 minutesNo. Floating at20 min% Floating at20 minutesDarkRoomLamp6. State a reasonable hypothesis that was tested in this experiment.7. Does the data collected support this hypothesis?8. Construct an Excel graph that would be used as figure to support this hypothesis9. If you repeated this experiment with using different colors of light (other wavelengths), what wouldyou predict as outcomes for blue, green and red light?D. Wavelength and Photosynthetic Rate10. In general, what is the effect of wavelength on the rate of oxygen production?11. Using the whole class data set (available on the web) create an Excel graph that relates lightcolor to oxygen production and wavelength.a) First let’s express oxygen production as a rate. Divide the mean oxygen production by thetotal time for the experiment, 10 minutes. What are the units for this rate?b) Now, examine the spectral analyses provided for each of the colored water used in thisexperiment. For each color choose a major transmitted wavelength range. c) Finally, create and Excel graph in which you show the absorbance spectrum of a typicalgreen plant pigment extract (from exercise B2 above) and the rate of oxygen production vs.wavelength. Suggestions for 11C above: - White light and darkness don’t fall on this graph, so do not use those data- Graph the data from B2 using a line graph (Wavelength will be on the x-axis and on one y-axis place the absorbance values- Use Oxygen Production in ml/min which you calculated in 11a.o On the other y-axis place the mean oxygen production values calculated from 11a.o You will need to have these three values at a particular wavelength associated withthe wavelengths from B2 in order to be able to graph them correctly. Please takenotes on how to do this.o You will fit the three bars to the oxygen productionColor of LightTotal O2Production(mg/L)Green Red Blue White DarkInitial O2Final O2Change


View Full Document
Download Laboratory Photosynthesis
Our administrator received your request to download this document. We will send you the file to your email shortly.
Loading Unlocking...
Login

Join to view Laboratory Photosynthesis and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Laboratory Photosynthesis 2 2 and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?