Electrophysiology on the Leech Hirudo Verbana medicinal leech Brandon Stevenson Francisco Madamba Example of P Cell Volts 10 Time Seconds Example of Synapse Volts 10 Time Seconds Receptors thought to play a role in learning Glutamate receptors and synaptic plasticity Voglis and Tavernarakis 2006 STDP in Mammals Dan and Poo 2005 STDP in leech Grey and Burrell 2010 Hurdo verbana Ventral Face of Leech Ganglion Anterior Pagoda cell AP cell Pressure cells P cells Todd 2010 Anterior Pagoda cell AP cell Pressure cells P cells AP cell AP cell P cells P cells Todd 2010 Gu 1990 How to sort out between the 400 different ganglions 1 Whether the cell fires tonically or not 2 The characteristic resting membrane potential RMP and the characteristic action potential AP of the cell which includes AP amplitude duration and frequency Todd 2010 Plan 1 Single cell electrophysiology 2 Dual cell electrophysiology Todd 2010 Application of STDP Volts Time Seconds Long Term Potentation EPSP Amplitude Volts Time Seconds Long Term Depression EPSP Amplitude millivolts Time Seconds How does our data compare Mammals Bi and Poo 1998 Difficulties 1 Insufficient Time 1 Rig set up 2 Ganglion dissection 3 Matlab usage 2 Equipment 1 Noise 3 Exact quantification of variables 1 Time 2 Hyperpolarization 4 Reliable Spikes 1 Not always getting spikes 1 Had to change extent of hyerpolarization
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