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Whole Blood HIV-1 Antibody by OraQuick



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U M D N J R O B E RT W J O H N S O N M E D I C A L S C H O O L P O C T P R O G RA M Chapter 10 Whole Blood HIV 1 Antibody by OraQuick CHAPTER 10 ORAQUICK Q U A L I TAT I V E HIV 1 1 of 7 SCREENING BY POCT Program PROCEDURE Procedure Whole Blood anti HIV Antibody by OraQuick PRINCIPLE The OraQuick Rapid HIV 1 Antibody Test is a single use qualitative immunoassay to detect antibodies to Human Immunodeficiency Virus Type 1 HIV 1 in fingerstick whole blood specimens The OraQuick is a manually performed visually read 20 40 minute immunoassay system It is comprised of a single use test device and a dingle use vial containing a premeasured amount of a buffered developer solution The OraQuick test utilizes a lateral flow procedure The assay test strip contains synthetic peptides representing the HIV envelope region in the Test zone and a goat anti human IgG procedural control in the Control zone immobilized onto a nitrocellulose membrane A fingerstick whole blood specimen is collected and transferred into a vial of developer solution followed by insertion of the test device into the developer vial The developer solution facilitates the flow of the specimen into the device and onto the test strip As the diluted specimen flows through the devices it rehydrates the protein A gold colorimetric reagent contained in the device As the specimen continues to migrate up the strip it encounters the T zone If the specimen contains antibodies that react with the antigens immobilized on the nitrocellulose membrane a reddish purple line will appear indicating the presence of antibodies to HIV 1 in the specimen Further up the strip the sample will encounter the C zone A reddish purple line at the C zone serves to demonstrate that a specimen containing antibodies was added to the vial and that the fluid has migrated adequately through the test device CLINICAL SIGNIFICANCE Acquired Immune Deficiency Syndrome AIDS Aids related complex ARC and pre AIDS are thought to be caused by the Human



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