BOLOGY 107 Lecture 6 Outline of Last Lecture I. Macromolecules (cont.)a. Implications of protein sequencingOutline of Current Lecture II. Cell Biologya. Microscopyb. Prokaryotesc. EukaryotesCurrent LectureCell Biology1) The basic unit of lifea) Made from macromolecules2) Microscopy formsa)Light Microscopy (LM)i)Visible light passed through, reflected off of, or emitted by the specimenii)Can use live cellsb)Electron Microscopy (EM)i)Electrons passed through or reflected off of the specimenii)Only dead cellsiii)Scanning Electron Microscopy (SEM)(1)Electrons bounced off of surface(2)Results in a 3-D imageiv)Transmission Electron Microscopy (TEM)(1)Electrons shot through specimen(2)Results in cross sectionv)Freeze Fracture Electron Microscopy(1)Freeze cells in ice, chip the ice(2)Splits the cell membrane into the two layers of phospholipidsc)Fractionationi)Use homogenization and centrifugation to create a pellet of specific cells3)Prokaryote- “before kernel”a)No nucleus, DNA is in nucleoid regionb)Possess a cell wallc)No membrane-bound organellesd)Plasma membraneThese notes represent a detailed interpretation of the professor’s lecture. GradeBuddy is best used as a supplement to your own notes, not as a substitute.e)Cytosolf)Chromosomesg)Ribosomes4)Eukaryotes- “true kernel”a)Plasma membraneb)Cytosol c)Chromosomesd)Ribosomese)Nucleusf)Only some cell have a cell wallg)Animal cellsi)Lysosomesii)Centrosomesiii)Flagellah)Plant cellsi)Chloroplastsii)Central vacuoleiii)Cell
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