DOC PREVIEW
UCLA CHEM 14CL - Guidelines

This preview shows page 1 out of 2 pages.

Save
View full document
View full document
Premium Document
Do you want full access? Go Premium and unlock all 2 pages.
Access to all documents
Download any document
Ad free experience
Premium Document
Do you want full access? Go Premium and unlock all 2 pages.
Access to all documents
Download any document
Ad free experience

Unformatted text preview:

This is a GROUP REPORTPost Lab ReportIsolationChem 14CLFall 2005This is a GROUP REPORTPost Lab ReportChem 14CL Fall 2005 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Post-lab Report Guidelines The post-lab report must be written inside your lab notebook (except graphs) THIS IS A GROUP REPORT POST LAB REPORT (I) ABSTRACT – Summarize the goal(s) of the experiment plus a summary of your experimental results (i.e. identities of components and their Rf values etc.) (II) OBSERVATIONS Isolation Rewrite all your observations during the extraction of the pigment and the chromatographic separation in an organized format. In tabular format record the number of the fraction being collected, the time over which the fraction is collected, the composition of the solvent used in the column (i.e. the mobile phase used), the appearance of colors on the column, and the color of the solution in the fraction being collected. Characterization Attach the ORIGINAL TLC plate in your report. In tabular format record the color of each component separated on the TLC plate, and its corresponding Rf value. (III) DATA ANALYSIS Identify the absorption signal(s) in the UV spectra that give rise to the colors of the chlorophyll and beta-carotene solutions (refer to the table listed below for colors of various components) Based on the absorption signals you identified, explain whether the locations of the absorptions make physical sense. Calculate the Rf values for all the components on the TLC plate. Show all your work. Using the guide below, identify as many spots on the TLC plate as you can. Determine which pigments were present in the yellow band and which were present in the green band from your column. Pigments in spinach in order of decreasing Rf values: Carotenes (1 spot) (yellow-orange) Pheophytin a (gray, may be nearly as intense as chlorophyll b) Pheophytin b (gray, may not be visible) Chlorophyll a (blue-green, more intense than chlorophyll b) Chlorophyll b (green) Xanthophylls (possibly 3 spots: yellow) 12 Chem 14CL Fall 2005 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Post-lab Report Guidelines The post-lab report must be written inside your lab notebook (except graphs) THIS IS A GROUP REPORT POST LAB REPORT (IV) CONCLUSIONS In your conclusion address how well the column separated the various pigments based on the following results. (i) Compare the separation of components on the TLC plate. (ii) Based on the locations of the absorption bands on the UV-Vis spectra Attach the ORIGINAL EXPERIMENTAL TLC plate with this post-lab report and turn in the completed report to your TA on the due date listed on the lab


View Full Document

UCLA CHEM 14CL - Guidelines

Documents in this Course
exp11

exp11

2 pages

Load more
Download Guidelines
Our administrator received your request to download this document. We will send you the file to your email shortly.
Loading Unlocking...
Login

Join to view Guidelines and access 3M+ class-specific study document.

or
We will never post anything without your permission.
Don't have an account?
Sign Up

Join to view Guidelines 2 2 and access 3M+ class-specific study document.

or

By creating an account you agree to our Privacy Policy and Terms Of Use

Already a member?